An automated confocal micro-extensometer enables in vivo quantification of mechanical properties with cellular resolution.

Robinson, Sarah; Huflejt, Michal; Barbier de Reuille, Pierre; Braybrook, Siobhan A; Schorderet, Martine; Reinhardt, Didier; Kuhlemeier, Cris (2017). An automated confocal micro-extensometer enables in vivo quantification of mechanical properties with cellular resolution. The Plant Cell, 29(10), tpc.00753.2017. American Society of Plant Biologists 10.1105/tpc.17.00753

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How complex developmental-genetic networks are translated into organs with specific 3D shapes remains an open question. This question is particularly challenging because the elaboration of specific shapes is in essence a question of mechanics. In plants, this means how the genetic circuitry affects the cell wall. The mechanical properties of the wall and their spatial variation are the key factors controlling morphogenesis in plants. However, these properties are difficult to measure and investigating their relation to genetic regulation is particularly challenging. To measure spatial variation of mechanical properties, one must determine the deformation of a tissue in response to a known force with cellular resolution. Here we present an automated confocal micro-extensometer (ACME), which greatly expands the scope of existing methods for measuring mechanical properties. Unlike classical extensometers, ACME is mounted on a confocal microscope and utilizes confocal images to compute the deformation of the tissue directly from biological markers, thus providing 3D cellular scale information and improved accuracy. Additionally, ACME is suitable for measuring the mechanical responses in live tissue. As a proof of concept, we demonstrate that the plant hormone gibberellic acid induces a spatial gradient in mechanical properties along the length of the Arabidopsis hypocotyl.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Robinson, Sarah Jane; Huflejt, Michal; Barbier de Reuille, Pierre; Braybrook, Siobhan; Reinhardt, Didier and Kuhlemeier, Cris

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

1040-4651

Publisher:

American Society of Plant Biologists

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

05 Dec 2017 09:11

Last Modified:

10 Dec 2017 02:23

Publisher DOI:

10.1105/tpc.17.00753

Additional Information:

unpublished preprint: https://boris.unibe.ch/106844/

BORIS DOI:

10.7892/boris.107619

URI:

https://boris.unibe.ch/id/eprint/107619

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