SERCA directs cell migration and branching across species and germ layers.

Bower, Danielle Vera; Lansdale, Nick; Navarro, Sonia; Truong, Thai V; Bower, Daniel James; Featherstone, Neil C; Connell, Marilyn G; Al Alam, Denise; Frey, Mark R; Trinh, Le A; Fernandez, G Esteban; Warburton, David; Fraser, Scott E; Bennett, Daimark; Jesudason, EC (2017). SERCA directs cell migration and branching across species and germ layers. Biology open BiO, 6(10), pp. 1458-1471. Company of Biologists 10.1242/bio.026039

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Branching morphogenesis underlies organogenesis in vertebrates and invertebrates, yet is incompletely understood. Here, we show that the sarco-endoplasmic reticulum Ca2+ reuptake pump (SERCA) directs budding across germ layers and species. Clonal knockdown demonstrated a cell-autonomous role for SERCA in Drosophila air sac budding. Live imaging of Drosophila tracheogenesis revealed elevated Ca2+ levels in migratory tip cells as they form branches. SERCA blockade abolished this Ca2+ differential, aborting both cell migration and new branching. Activating protein kinase C (PKC) rescued Ca2+ in tip cells and restored cell migration and branching. Likewise, inhibiting SERCA abolished mammalian epithelial budding, PKC activation rescued budding, while morphogens did not. Mesoderm (zebrafish angiogenesis) and ectoderm (Drosophila nervous system) behaved similarly, suggesting a conserved requirement for cell-autonomous Ca2+ signaling, established by SERCA, in iterative budding.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Institute of Diagnostic, Interventional and Paediatric Radiology
10 Strategic Research Centers > Center for Space and Habitability (CSH)

UniBE Contributor:

Bower, Danielle Vera and Bower, Daniel James

Subjects:

600 Technology > 610 Medicine & health
500 Science > 520 Astronomy

ISSN:

2046-6390

Publisher:

Company of Biologists

Language:

English

Submitter:

Nicole Rösch

Date Deposited:

23 Feb 2018 15:40

Last Modified:

28 Oct 2019 22:02

Publisher DOI:

10.1242/bio.026039

PubMed ID:

28821490

Uncontrolled Keywords:

Branching morphogenesis Calcium dynamics Cell migration SERCA

BORIS DOI:

10.7892/boris.107764

URI:

https://boris.unibe.ch/id/eprint/107764

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