Characterization of a putative MICOS complex in Trypanosoma brucei

Schimanski, Bernd (9 October 2017). Characterization of a putative MICOS complex in Trypanosoma brucei (Unpublished). In: EMBO-FEBS Lecture Course: Mitochondria in life, death and disease. Selva di Fasano (BR), Italy. 09.10.17 - 13.10.17.

The mitochondrial contact site and cristae organizing system (MICOS) is crucial for the formation and maintenance of cristae junctions in the inner boundary membrane of mitochondria. This protein complex is well studied in eukaryotic model organisms beloning to the supergroup of Opisthokonta such as yeast and human. In Saccharomyces cerevisiae, the MICOS complex consists of at least six components with Mic60 and Mic10 as core subunits. We started to study a putative MICOS complex in the kinetoplastid parasite Trypanosoma brucei - an important pathogen belonging to the supergroup of Excavata. Of all described MICOS subunits only an ortholog of Mic10 can be identified in trypanosomes based on sequence similarity. We generated a knock-out cell line and could show that loss of TbMic10 leads to a significant lower number of cristae in the single mitochondrion compared to wild-type cells. Immunoprecipitation (IP) of epitope-tagged TbMic10 combined with SILAC (stable isotope labeling by amino acids in cell culture) and quantitative mass spectrometry led to the identification of further putative MICOS candidates. From those, two additional bait proteins were selected for SILAC-IPs which allowed us to define a set of proteins that might represent the subunits of a putative MICOS complex in trypanosomes. All candidates show mitochondrial localization and seem to form a stable high molecular weight complex. Our results indicate that this complex is highly divergent from known MICOS complexes of higher eukaryotes.

Item Type:

Conference or Workshop Item (Poster)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Schimanski, Bernd

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

Language:

English

Submitter:

Christina Schüpbach

Date Deposited:

23 Jan 2018 13:26

Last Modified:

23 Jan 2018 13:26

URI:

https://boris.unibe.ch/id/eprint/108279

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