Comparative analysis of DNA methylation patterns of equine sarcoid and healthy skin samples

Semik, E.; Ząbek, T.; Gurgul, A.; Fornal, A.; Szmatoła, T.; Pawlina, K.; Wnuk, M.; Klukowska-Rötzler, Jolanta; Koch, Christoph; Mählmann, K.; Bugno-Poniewierska, M. (2018). Comparative analysis of DNA methylation patterns of equine sarcoid and healthy skin samples. Veterinary and comparative oncology, 16(1), pp. 37-46. Wiley-Blackwell 10.1111/vco.12308

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OBJECTIVE:
In this study, for the first time we report the genome-wide DNA methylation profile of skin tumour in horses and describe differentially methylated genomic regions (DMRs) with respect to healthy skin.
MATERIALS & METHODS:
The comparative analysis of DNA methylation patterns detected using Reduced Representation Bisulfite Sequencing (RRBS) technique, allowed identification of 136 regions showing differential methylation between sarcoid and normal skin tissue.
RESULTS:
Most of the identified DMRs were short fragments, less than 1 kb in size, located in the intergenic regions. Among identified DMRs there were also regions located within genes directly or indirectly related with oncogenesis. We additionally validated 9 CpG sites showing hypomethylation and 9 CpG sites that were hypermethylated in lesional sample, confirming the identified changes in the DNA methylation.
CONCLUSION:
Knowledge on the changes taking place in the process of DNA methylation may provide a basis for the development of new alternative diagnostic or therapeutic approaches to equine sarcoids.

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