Comparative analysis of DNA methylation patterns of equine sarcoid and healthy skin samples

Semik, E.; Ząbek, T.; Gurgul, A.; Fornal, A.; Szmatoła, T.; Pawlina, K.; Wnuk, M.; Klukowska-Rötzler, Jolanta; Koch, Christoph; Mählmann, K.; Bugno-Poniewierska, M. (2018). Comparative analysis of DNA methylation patterns of equine sarcoid and healthy skin samples. Veterinary and comparative oncology, 16(1), pp. 37-46. Wiley-Blackwell 10.1111/vco.12308

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OBJECTIVE: In this study, for the first time we report the genome-wide DNA methylation profile of skin tumour in horses and describe differentially methylated genomic regions (DMRs) with respect to healthy skin. MATERIALS & METHODS: The comparative analysis of DNA methylation patterns detected using Reduced Representation Bisulfite Sequencing (RRBS) technique, allowed identification of 136 regions showing differential methylation between sarcoid and normal skin tissue. RESULTS: Most of the identified DMRs were short fragments, less than 1 kb in size, located in the intergenic regions. Among identified DMRs there were also regions located within genes directly or indirectly related with oncogenesis. We additionally validated 9 CpG sites showing hypomethylation and 9 CpG sites that were hypermethylated in lesional sample, confirming the identified changes in the DNA methylation. CONCLUSION: Knowledge on the changes taking place in the process of DNA methylation may provide a basis for the development of new alternative diagnostic or therapeutic approaches to equine sarcoids.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > DermFocus
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Equine Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Hämatologie / Onkologie (Pädiatrie)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Hämatologie / Onkologie (Pädiatrie)

UniBE Contributor:

Klukowska-Rötzler, Jolanta and Koch, Christoph

Subjects:

600 Technology > 630 Agriculture

ISSN:

1476-5810

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Andrea Gassmann-Suter

Date Deposited:

19 Apr 2018 13:42

Last Modified:

16 Aug 2018 15:39

Publisher DOI:

10.1111/vco.12308

PubMed ID:

28220614

BORIS DOI:

10.7892/boris.108413

URI:

https://boris.unibe.ch/id/eprint/108413

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