Gene Network Analysis of Interstitial Macrophages After Treatment with Induced Pluripotent Stem Cells Secretome (iPSC-cm) in the Bleomycin Injured Rat Lung.

Tamò, Luca Giuseppe Athos; Simillion, Cedric; Hibaoui, Youssef; Feki, Anis; Gugger, Mathias; Prasse, Antje; Jäger, Benedikt; Goldmann, Torsten; Geiser, Thomas; Gazdhar, Amiq (2018). Gene Network Analysis of Interstitial Macrophages After Treatment with Induced Pluripotent Stem Cells Secretome (iPSC-cm) in the Bleomycin Injured Rat Lung. Stem cell reviews and reports, 14(3), pp. 412-424. Springer 10.1007/s12015-017-9790-9

[img]
Preview
Text
10.1007%2Fs12015-017-9790-9.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (4MB) | Preview

Idiopathic pulmonary fibrosis (IPF) is a complex disease involving various cell types. Macrophages are essential in maintenance of physiological homeostasis, wound repair and fibrosis in the lung. Macrophages play a crucial role in repair and remodeling by altering their phenotype and secretory pattern in response to injury. The secretome of induced pluripotent stem cells (iPSC-cm) attenuates injury and fibrosis in bleomycin injured rat lungs. In the current study, we evaluate the effect of iPSC-cm on gene expression and phenotype of interstitial macrophage in bleomycin injured rat lungs in vivo. iPSC-cm was intratracheally instilled 7 days after bleomycin induced lung injury and assessed 7 days later and single cell isolation was performed. Macrophages were FACS sorted and microarray analysis was performed. We characterized changes in the rat lung interstitial macrophages using transcriptional profiling. iPSC-cm reduced the total collagen content of the lung and reduced different macrophage populations. Gene set enrichment analysis revealed involvement of three essential pathways (a) immune modulation, (b) branching morphogenesis and (c) canonical Wnt signaling. This study demonstrates that iPSC-cm reduces fibrosis in bleomycin injured rat lung by partially altering the macrophages and regulating their gene expression.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology

UniBE Contributor:

Tamò, Luca Giuseppe Athos; Geiser, Thomas and Gazdhar, Amiq

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1550-8943

Publisher:

Springer

Language:

English

Submitter:

Rahel Holderegger

Date Deposited:

18 Jan 2018 11:08

Last Modified:

22 May 2018 01:30

Publisher DOI:

10.1007/s12015-017-9790-9

PubMed ID:

29256173

Uncontrolled Keywords:

Induced pluripotent stem cells Lung fibrosis Macrophages Stem cell secretome

BORIS DOI:

10.7892/boris.108530

URI:

https://boris.unibe.ch/id/eprint/108530

Actions (login required)

Edit item Edit item
Provide Feedback