Electrophysiological Recordings on a Sounding Rocket: Report of a First Attempt Using Xenopus laevis Oocytes

Wüest, Simon; Plüss, Tobias; Hardegger, Christoph; Felder, Mario; Kunz, Aaron; Fleischli, Benno; Komotar, Carlos; Rüdlinger, Lukas; Albisser, Andreas; Gisler, Thomas; Frauchiger, Daniela Angelika; Egli, Marcel (2017). Electrophysiological Recordings on a Sounding Rocket: Report of a First Attempt Using Xenopus laevis Oocytes. Gravitational and Space Research, 5(2), pp. 43-56.

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It is not fully understood how cells detect external mechanical forces, but mechanosensitive ion channels play important roles in detecting and translating physical forces into biological responses (mechanotrans-duction). With the “OoClamp” device, we developed a tool to study electrophysiological processes, including the gating properties of ion channels under various gravity conditions. The “OoClamp” device uses an adapted patch clamp technique and is operational during parabolic flight and centrifugation up to 20 g. In the framework of the REXUS/BEXUS program, we have further developed the “OoClamp” device with the goal of conducting electrophysiological experiments aboard a flying sounding rocket.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute for Surgical Technology & Biomechanics ISTB

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Wüest, Simon and Frauchiger, Daniela Angelika

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2332-7774

Funders:

[UNSPECIFIED] Swiss Space Office

Language:

English

Submitter:

Simon Wüest

Date Deposited:

24 Jan 2018 09:41

Last Modified:

24 Jan 2018 09:41

Uncontrolled Keywords:

Electrophysiology, Microgravity, Xenopus laevis Oocytes, Sounding Rocket, Ion Mechanosensitive Mechanobiology Channels

BORIS DOI:

10.7892/boris.108566

URI:

https://boris.unibe.ch/id/eprint/108566

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