Roth, Beat; Jayaratna, Isuru; Sundi, Debasish; Cheng, Tiewei; Melquist, Jonathan; Choi, Woonyoung; Porten, Sima; Nitti, Giovanni; Navai, Neema; Wszolek, Matthew; Guo, Charles; Czerniak, Bogdan; McConkey, David; Dinney, Colin (2017). Employing an orthotopic model to study the role of epithelial-mesenchymal transition in bladder cancer metastasis. OncoTarget, 8(21), pp. 34205-34222. Impact Journals LLC 10.18632/oncotarget.11009
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Epithelial-to-mesenchymal transition (EMT) has been implicated in the progression of bladder cancer. To study its contribution to bladder cancer metastasis, we established new xenograft models derived from human bladder cancer cell lines utilizing an orthotopic "recycling" technique that allowed us to isolate and examine the primary tumor and its corresponding circulating tumor cells (CTC's) and metastatic lesions. Using whole genome mRNA expression profiling, we found that a reversible epithelial-to-mesenchymal transition (EMT) characterized by TGFβ pathway activation and SNAIL expression was associated with the accumulation of CTCs. Finally, we observed that conditional silencing of SNAIL completely blocked CTC production and regional/distant metastasis. Using this unique bladder cancer xenograft model, we conclude that metastasis is dependent on a reversible EMT mediated by SNAIL.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Urology |
UniBE Contributor: |
Roth, Beat |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1949-2553 |
Publisher: |
Impact Journals LLC |
Language: |
English |
Submitter: |
Laetitia Hayoz |
Date Deposited: |
01 Mar 2018 09:33 |
Last Modified: |
05 Dec 2022 15:09 |
Publisher DOI: |
10.18632/oncotarget.11009 |
PubMed ID: |
27494900 |
Uncontrolled Keywords: |
SNAIL bladder cancer circulating tumor cells metastasis orthotopic xenografts |
BORIS DOI: |
10.7892/boris.108826 |
URI: |
https://boris.unibe.ch/id/eprint/108826 |
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