Leukocyte-platelet aggregates-a phenotypic characterization of different stages of peripheral arterial disease.

Dopheide, Jörn Fredrik; Rubrech, Jennifer; Trumpp, Amelie; Geissler, Philip; Zeller, Geraldine C; Bock, Karsten; Dünschede, Friedrich; Trinh, Tran Tong; Dorweiler, Bernhard; Münzel, Thomas; Radsak, Markus P; Espinola-Klein, Christine (2016). Leukocyte-platelet aggregates-a phenotypic characterization of different stages of peripheral arterial disease. Platelets, 27(7), pp. 658-667. Informa Healthcare 10.3109/09537104.2016.1153619

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The formation of monocyte-platelet aggregates and neutrophil-platelet aggregates (MPA and NPA, respectively) is influenced by inflammation, but also might contribute to an exacerbation of inflammatory responses in atherosclerotic plaque. The purpose of this study was to analyze MPA and NPA proportions in regard to different stages of peripheral arterial disease (PAD). Forty-five patients with intermittent claudication (IC) (3 groups: Rutherford (R)-1, R-2, and R-3; each n = 15), 20 patients with critical limb ischemia (CLI) (Rutherford 5 (40%) and 6 (60%)), and 20 healthy controls were studied. Analyses of monocyte (Mon) subpopulations (CD14++CD16- (classical) Mon1, CD14++CD16+ (intermediate) Mon2, CD14+CD16++ (non-classical) Mon3), MPA, and NPA was performed from whole blood by flow cytometry. Controls showed an increased proportion of the Mon1 subpopulation (p < 0.001), whereas CLI patients showed a significant increase of the Mon2 subpopulation compared to controls, R-1, or R-2 patients (p < 0.0001). For the Mon3 subpopulation, CLI and R-3 patients showed an increased proportion (p < 0.05). MPA formation with the proinflammatory Mon2 and Mon3 subpopulations was increased in CLI patients (both p < 0.01). Similarly, NPA was significantly increased in CLI patients (p < 0.05). Serological markers of inflammation and procoagulation (fibrinogen [r = 0.459, p < 0.001], soluble triggering receptor expressed on myeloid cells (sTREM-1) [r = 0.237, p < 0.05] and P-Selectin [r = 0.225, p < 0.05]) correlated directly with MPA formation on the Mon2 subpopulation. We found an association of inflammatory and procoagulatory markers with increased formation of MPA on the Mon2 subpopulation. Since R-3 patients also had significantly increased MPA, one can speculate that the inflammatory burden might promote an aggravation of the disease.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Angiology
UniBE Contributor:	Dopheide, Jörn Fredrik
Subjects:	600 Technology > 610 Medicine & health
ISSN:	0953-7104
Publisher:	Informa Healthcare
Language:	English
Submitter:	Catherine Gut
Date Deposited:	04 Jun 2018 15:02
Last Modified:	05 Dec 2022 15:09
Publisher DOI:	10.3109/09537104.2016.1153619
PubMed ID:	27352829
Uncontrolled Keywords:	Critical limb ischemia MPA NPA PMN TREM-1 monocytes peripheral arterial disease sTREM-1
URI:	https://boris.unibe.ch/id/eprint/108857