Phenotypic and functional characterization of neutrophils and monocytes from patients with myelodysplastic syndrome by flow cytometry.

Schmidt, Cornelia S; Aranda Lopez, Pamela; Dopheide, Jörn Fredrik; Schmidt, Frank; Theobald, Matthias; Schild, Hansjörg; Lauinger-Lörsch, Evi; Nolte, Florian; Radsak, Markus P (2016). Phenotypic and functional characterization of neutrophils and monocytes from patients with myelodysplastic syndrome by flow cytometry. Cellular immunology, 308, pp. 19-26. Elsevier 10.1016/j.cellimm.2016.07.005

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Myelodysplastic syndrome (MDS) is a clonal stem cell disorder frequently associated with inefficient granulopoiesis showing dysplastic polymorphonuclear neutrophils (PMNs). To assess PMN functionality in MDS in a clinical routine setting, 30 MDS patients and ten healthy volunteers were analyzed for PMN and monocyte phenotype and function (degranulation, CD62L shedding, oxidative burst and phagocytosis) upon stimulation with lipopolysaccharide by multi-color flow cytometry (MCFC). Our data show a heterogeneous pattern for CD66, CD16 and CD64 expression on PMNs of MDS patients. CD62L shedding rate and CD66 degranulation were reduced. Interestingly, we detected correlations between the WHO adapted prognostic scoring system (WPSS) and CD16 expression on PMNs as well as the international prognostic scoring system (IPSS) and CD11b degranulation by MCFC, suggesting clinical relevance of MCFC based function testing. In conclusion, MCFC of myelodysplastic immunophenotypes and PMN functionality are applicable in clinical settings, but further prospective studies are needed to assess the practical clinical value of such analyses.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Angiology

UniBE Contributor:

Dopheide, Jörn Fredrik

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0008-8749

Publisher:

Elsevier

Language:

English

Submitter:

Catherine Gut

Date Deposited:

04 Jun 2018 14:58

Last Modified:

21 Apr 2020 16:35

Publisher DOI:

10.1016/j.cellimm.2016.07.005

PubMed ID:

27417453

Uncontrolled Keywords:

Effector functions Monocytes Myelodysplastic syndrome Neutrophils Phenotype

BORIS DOI:

10.7892/boris.108860

URI:

https://boris.unibe.ch/id/eprint/108860

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