Effect of Clostridium perfringens β-Toxin on Platelets

Thiel, Anne; Mogel, Helga Maria; Bruggisser, Julia; Baumann, Arnaud; Wyder, Marianne; Stoffel, Michael Hubert; Summerfield, Artur; Posthaus, Horst (2017). Effect of Clostridium perfringens β-Toxin on Platelets. Toxins, 9(10), p. 336. MDPI 10.3390/toxins9100336

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Clostridium perfringensβ-toxin (CPB) is the major virulence factor of C.perfringens type C causing a hemorrhagic enteritis in animals and humans. In experimentally infected pigs, endothelial binding of CPB was shown to be associated with early vascular lesions and hemorrhage but without obvious thrombosis of affected vessels, suggesting altered hemostasis in the early phase of the disease. The objective of the present study was to investigate the effect of CPB on platelets, with respect to primary hemostasis. Our results demonstrate that CPB binds to porcine and human platelets and forms oligomers resulting in a time- and dose-dependent cell death. Platelets showed rapid ultrastructural changes, significantly decreased aggregation and could no longer be activated by thrombin. This indicates that CPB affects the physiological function of platelets and counteracts primary hemostasis. Our results add platelets to the list of target cells of CPB and extend the current hypothesis of its role in the pathogenesis of C. perfringens type C enteritis

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)
09 Interdisciplinary Units > Microscopy Imaging Center MIC

UniBE Contributor:

Thiel, Anne; Mogel, Helga Maria; Bruggisser, Julia; Baumann, Arnaud; Wyder, Marianne; Stoffel, Michael Hubert; Summerfield, Artur and Posthaus, Horst

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

2072-6651

Publisher:

MDPI

Language:

English

Submitter:

Artur Summerfield

Date Deposited:

02 May 2018 12:48

Last Modified:

11 Jan 2019 13:37

Publisher DOI:

10.3390/toxins9100336

PubMed ID:

29064418

BORIS DOI:

10.7892/boris.108899

URI:

https://boris.unibe.ch/id/eprint/108899

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