Increased capillary tortuosity and pericapillary basement membrane thinning in skeletal muscle of mice undergoing running wheel training.

Baum, Oliver; Sollberger, Carole; Raaflaub, Andrea; Odriozola Quesada, Adolfo; Spohr, Gunnar; Frese, Sebastian; Tschanz, Stefan A. (2018). Increased capillary tortuosity and pericapillary basement membrane thinning in skeletal muscle of mice undergoing running wheel training. Journal of Experimental Biology, 221(Pt 4) Company of Biologists 10.1242/jeb.171819

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To work out which microvascular remodeling processes occur in murine skeletal muscle during endurance exercise, we subjected C57BL/6-mice to voluntary running wheel training for 1 week (1wk-t) or 6 weeks (6wks-t). By means of morphometry, the capillarity as well as the compartmental and sub-compartmental structure of the capillaries were quantitatively described at the light microscopy and at the electron microscopy level, respectively, in the plantaris muscle (PLNT) of the exercising mice in comparison to untrained littermates. In the early phase of the training (1wk-t), angiogenesis (32%-higher capillary-fiber (CF)-ratio; P<0.05) in PLNT was accompanied by a tendency of capillary lumen enlargement (30%; P=0.06) and reduction of the pericapillary basement membrane thickness (CBMT; 12.7%; P=0.09) as well as a 21%-shortening of intraluminal protrusion length (P<0.05), all compared to controls. After long-term training (6wks-t), when the mice reached a steady state in running activity, additional angiogenesis (CF-ratio: 76%; P<0.05) and a 16.3%-increase in capillary tortuosity (P<0.05) were established, accompanied by reversal of the lumen expansion (23%; P>0.05), further reduction of CBMT (16.5%; P<0.05) and additional shortening of the intraluminal protrusion length (23%; P<0.05), all compared to controls. Other structural indicators such as capillary profile sizes, profile area densities, perimeters of the capillary compartments and concentrations of endothelium-pericyte peg-socket junctions were not significantly different between the mouse groups. Besides angiogenesis, increase of capillary tortuosity and reduction of CBMT represent the most striking microvascular remodeling processes in skeletal muscle of mice that undergo running wheel training.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
09 Interdisciplinary Units > Microscopy Imaging Center MIC

UniBE Contributor:

Odriozola Quesada, Adolfo and Tschanz, Stefan A.

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0022-0949

Publisher:

Company of Biologists

Language:

English

Submitter:

Stefan Andreas Tschanz

Date Deposited:

16 Jan 2018 16:32

Last Modified:

14 Feb 2019 13:51

Publisher DOI:

10.1242/jeb.171819

PubMed ID:

29246972

Uncontrolled Keywords:

Capillaries Endurance exercise Morphometry Mouse Skeletal muscle Transmission electron microscopy

BORIS DOI:

10.7892/boris.108947

URI:

https://boris.unibe.ch/id/eprint/108947

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