Ultrastructure of Skeletal Muscles in Mice Lacking Muscle-Specific VEGF Expression.

Baum, Oliver; Jentsch, Lena; Odriozola Quesada, Adolfo; Tschanz, Stefan A.; Olfert, I Mark (2017). Ultrastructure of Skeletal Muscles in Mice Lacking Muscle-Specific VEGF Expression. Anatomical record: advances in integrative anatomy and evolutionary biology, 300(12), pp. 2239-2249. John Wiley & Sons, Inc. 10.1002/ar.23644

[img] Text
Baum_et_al-2017-The_Anatomical_Record.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (1MB) | Request a copy

Vascular endothelial growth factor-A (VEGF) influences several physiological processes including endothelial cell function, angiogenesis and maintenance of organ/tissue capillarity. While the functional aspects of VEGF were vigorously investigated, only little detail is known on structural integrity of skeletal muscle fibers and capillaries in mice lacking VEGF expression in their muscles. Therefore, we assessed systematically the architecture of the glycolytic plantaris and the oxidative soleus muscles obtained from muscle-specific VEGF knockout (mVEGF-KO, n = 7) mice and their wild-type (WT, n = 7) littermates by morphometry after transmission electron microscopy. The capillary/fiber ratio was lower (plantaris: -63.5%; soleus: -54.8%; P ≤ 0.05) in mVEGF-KO mice than in WT mice. In plantaris, quantification of volume density (Vv) of compartments revealed higher Vv of total mitochondria (+56.5%, P ≤ 0.05) as well as higher Vv-values for both intrafibrillar (+39%; P ≤ 0.05) and subsarcolemmal (+220%; P ≤ 0.05) mitochondrial pools in mVEGF-KO mice than WT mice. The capillary phenotype also differed (P ≤ 0.05) between the two mouse-strains: Vv (-17.4%), absolute area size (-19.1%) and thickness (-19.6%) of the endothelium layer were lower and Vv of capillary lumen (+15.1%) was higher in mVEGF-KO mice than in WT littermates. In soleus, mitochondrial Vv in fibers and the structural indicators specific to the capillary phenotype exhibited the same tendency in differences between the mouse strains without reaching statistical significance. Our morphometric analysis demonstrates that the lower capillary supply in plantaris of mVEGF-KO mice is accompanied by higher mitochondrial Vv in muscle fibers as well as lumen dilation and endothelium thinning of capillaries. These structural alterations were more pronounced in a glycolytic than an oxidative muscle. Anat Rec, 300:2239-2249, 2017. © 2017 Wiley Periodicals, Inc.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
09 Interdisciplinary Units > Microscopy Imaging Center MIC

UniBE Contributor:

Odriozola Quesada, Adolfo and Tschanz, Stefan A.

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1932-8486

Publisher:

John Wiley & Sons, Inc.

Language:

English

Submitter:

Stefan Andreas Tschanz

Date Deposited:

17 Jan 2018 12:41

Last Modified:

14 Feb 2019 13:52

Publisher DOI:

10.1002/ar.23644

PubMed ID:

28710834

Uncontrolled Keywords:

morphometry skeletal muscle capillaries transmission electron microscopy

BORIS DOI:

10.7892/boris.108949

URI:

https://boris.unibe.ch/id/eprint/108949

Actions (login required)

Edit item Edit item
Provide Feedback