Comprehensive characteristics of microRNA expression profile of equine sarcoids

Pawlina, Klaudia; Gurgul, Artur; Szmatoła, Tomasz; Koch, Christoph; Mählmann, Kathrin; Witkowski, Maciej; Bugno-Poniewierska, Monika (2017). Comprehensive characteristics of microRNA expression profile of equine sarcoids. Biochimie, 137, pp. 20-28. Elsevier Masson SAS 10.1016/j.biochi.2017.02.017

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Equine sarcoids are the most common neoplasms occurring in horses. Despite frequent occurrence, they are still not well described at the molecular level. Thus, in the present study, we performed a comprehensive comparative analysis of sarcoid miRNAome profile to identify aberrantly expressed microRNAs, along with their structural variants, potentially useful as biomarkers and, in a wider perspective, broaden the knowledge about this tumor and underlying mechanisms. To this end, we conducted next generation sequencing and as a result we identified both known and potentially novel miRNAs. Differential expression analysis revealed the existence of almost one hundred miRNAs being over- or underexpressed in sarcoids in comparison to healthy tissue (p-adj<0.05), of which many are known for their involvement in processes crucial for neoplastic transformation. Among upregulated miRNAs there were those associated with decreased cell adhesion abilities as well as engaged in global protein production, while downregulation of some miRs i.a. increased cell expansion abilities. Moreover, we identified altered expression levels of miRNA variants (isomiRs) between the investigated tissues. Further analysis revealed that 5' isomiRs comprise different seed sequences leading to target gene switching followed by activation of different biological pathways. Our results are the first which revealed the complexity of microRNA profiles in equine sarcoids and skin tissue, along with the dynamism of their growing in importance concomitants, namely isomiRs. They also showed miRNA molecules and biological pathways important from the sarcoid oncogenesis point of view.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > DermFocus
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Equine Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)

UniBE Contributor:

Koch, Christoph

Subjects:

600 Technology > 630 Agriculture

ISSN:

0300-9084

Publisher:

Elsevier Masson SAS

Language:

English

Submitter:

Andrea Gassmann-Suter

Date Deposited:

02 May 2018 12:53

Last Modified:

16 Oct 2018 15:26

Publisher DOI:

10.1016/j.biochi.2017.02.017

PubMed ID:

28259757

BORIS DOI:

10.7892/boris.109019

URI:

https://boris.unibe.ch/id/eprint/109019

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