A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement

Thomsen, Andreas R.; Aldrian, Christine; Bronsert, Peter; Thomann, Yi; Nanko, Norbert; Melin, Nicolas; Rücker, Gerta; Follo, Marie; Grosu, Anca L.; Niedermann, Gabriele; Layer, Paul G.; Heselich, Anja; Lund, Per G. (2018). A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement. Lab on a chip, 18(1), pp. 179-189. Royal Society of Chemistry 10.1039/C7LC00832E

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Multicellular spheroids represent a well-established 3D model to study healthy and diseased cells in vitro. The use of conventional 3D cell culture platforms for the generation of multicellular spheroids is limited to cell types that easily self-assemble into spheroids because less adhesive cells fail to form stable aggregates. A high-precision micromoulding technique developed in our laboratory produces deep conical agarose microwell arrays that allow the cultivation of uniform multicellular aggregates, irrespective of the spheroid formation capacity of the cells. Such hydrogel arrays warrant a steady nutrient supply for several weeks, permit live volumetric measurements to monitor cell growth, enable immunohistochemical staining, fluorescence-based microscopy, and facilitate immediate harvesting of cell aggregates. This system also allows co-cultures of two distinct cell types either in direct cell–cell contact or at a distance as the hydrogel permits diffusion of soluble compounds. Notably, we show that co-culture of a breast cancer cell line with bone marrow stromal cells enhances 3D growth of the cancer cells in this system.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Viszeralchirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Viszeralchirurgie

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Radiation Oncology

UniBE Contributor:

Melin, Nicolas

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1473-0197

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Lilian Karin Smith-Wirth

Date Deposited:

09 Apr 2018 13:41

Last Modified:

16 May 2021 00:16

Publisher DOI:

10.1039/C7LC00832E

PubMed ID:

29211089

BORIS DOI:

10.7892/boris.109070

URI:

https://boris.unibe.ch/id/eprint/109070

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