Transposon-associated lincosamide resistance lnu (C) gene identified in Brachyspira hyodysenteriae ST83

De Luca, Silvio; Nicholson, Pamela; Magistrali, Chiara F.; Garcia Martin, Ana Bélen; Rychener, Lorenz; Zeeh, Friederike; Frey, Joachim; Perreten, Vincent (2018). Transposon-associated lincosamide resistance lnu (C) gene identified in Brachyspira hyodysenteriae ST83. Veterinary microbiology, 214, pp. 51-55. Elsevier 10.1016/j.vetmic.2017.12.003

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Treatment of Swine Dysentery (SD) caused by Brachyspira hyodysenteriae (B. hyodysenteriae) is carried out using antimicrobials such as macrolides, lincosamides and pleuromutilins leading to the selection of resistant strains. Whole genome sequencing of a multidrug-resistant B. hyodysenteriae strain called BH718 belonging to sequence type (ST) 83 revealed the presence of the lincosamide resistance gene lnu(C) on the small 1724-bp transposon MTnSag1. The strain also contains an A to T substitution at position 2058 (A2058T) in the 23S rRNA gene which is known to be associated with macrolide and lincosamide resistance in B. hyodysenteriae. Testing of additional strains showed that those containing lnu(C) exhibited a higher minimal inhibitory concentration (MIC) of lincomycin (MIC ≥ 64 mg/L) compared to strains lacking lnu(C), even if they also harbor the A2058T mutation. Resistance to pleuromutilins could not be explained by the presence of already reported mutations in the 23S rRNA gene and in the ribosomal protein L3. This study shows that B. hyodysenteriae has the ability to acquire mobile genetic elements conferring resistance to antibiotics.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Swine Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

De Luca, Silvio; Nicholson, Pamela; Garcia Martin, Ana Bélen; Rychener, Lorenz; Zeeh, Friederike; Frey, Joachim and Perreten, Vincent

Subjects:

600 Technology > 630 Agriculture

ISSN:

0378-1135

Publisher:

Elsevier

Language:

English

Submitter:

Daniela Fasel

Date Deposited:

10 Apr 2018 16:38

Last Modified:

11 Jan 2019 13:02

Publisher DOI:

10.1016/j.vetmic.2017.12.003

PubMed ID:

29408032

BORIS DOI:

10.7892/boris.109100

URI:

https://boris.unibe.ch/id/eprint/109100

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