Effects of gasoline and ethanol-gasoline exhaust exposure on human bronchial epithelial and natural killer cells in vitro.

Roth, Michèle; Usemann, Jakob; Bisig, Christoph; Comte, Pierre; Czerwinski, Jan; Mayer, Andreas C R; Beier, Konstantin; Rothen-Rutishauser, Barbara; Latzin, Philipp; Müller, Loretta Lina (2017). Effects of gasoline and ethanol-gasoline exhaust exposure on human bronchial epithelial and natural killer cells in vitro. Toxicology in vitro, 45(Pt 1), pp. 101-110. Elsevier 10.1016/j.tiv.2017.08.016

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Air pollution exposure, including passenger car emissions, may cause substantial respiratory health effects and cancer death. In western countries, the majority of passenger cars are driven by gasoline fuel. Recently, new motor technologies and ethanol fuels have been introduced to the market, but potential health effects have not been thoroughly investigated. We developed and verified a coculture model composed of bronchial epithelial cells (ECs) and natural killer cells (NKs) mimicking the human airways to compare toxic effects between pure gasoline (E0) and ethanol-gasoline-blend (E85, 85% ethanol, 15% gasoline) exhaust emitted from a flexfuel gasoline car. We drove a steady state cycle, exposed ECs for 6h and added NKs. We assessed exhaust effects in ECs alone and in cocultures by RT-PCR, flow cytometry, and oxidative stress assay. We found no toxic effects after exposure to E0 or E85 compared to air controls. Comparison between E0 and E85 exposure showed a weak association for less oxidative DNA damage after E85 exposure compared to E0. Our results indicate that short-term exposure to gasoline exhaust may have no major toxic effects in ECs and NKs and that ethanol as part of fuel for gasoline cars may be favorable.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Pneumologie (Pädiatrie)
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine > Paediatric Pneumology

UniBE Contributor:

Latzin, Philipp and Müller, Loretta Lina

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0887-2333

Publisher:

Elsevier

Language:

English

Submitter:

Romy Melanie Rodriguez Del Rio

Date Deposited:

12 Feb 2018 10:52

Last Modified:

04 Nov 2019 14:30

Publisher DOI:

10.1016/j.tiv.2017.08.016

PubMed ID:

28843492

Uncontrolled Keywords:

Airways Cocultures DNA damage Diesel exhaust particles Ethanol-gasoline blend Flexfuel Gasoline exhaust

BORIS DOI:

10.7892/boris.109247

URI:

https://boris.unibe.ch/id/eprint/109247

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