The naringenin-induced exoproteome of Rhizobium etli CE3.

Meneses Moreno, Niurka; Taboada, Hermenegildo; Dunn, Michael F; Vargas, María Del Carmen; Buchs, Natasha; Heller, Manfred; Encarnación, Sergio (2017). The naringenin-induced exoproteome of Rhizobium etli CE3. Archives of microbiology, 199(5), pp. 737-755. Springer 10.1007/s00203-017-1351-8

10.1007%2Fs00203-017-1351-8.pdf - Published Version
Available under License Publisher holds Copyright.

Download (994kB) | Preview

Flavonoids excreted by legume roots induce the expression of symbiotically essential nodulation (nod) genes in rhizobia, as well as that of specific protein export systems. In the bean microsymbiont Rhizobium etli CE3, nod genes are induced by the flavonoid naringenin. In this study, we identified 693 proteins in the exoproteome of strain CE3 grown in minimal medium with or without naringenin, with 101 and 100 exoproteins being exclusive to these conditions, respectively. Four hundred ninety-two (71%) of the extracellular proteins were found in both cultures. Of the total exoproteins identified, nearly 35% were also present in the intracellular proteome of R. etli bacteroids, 27% had N-terminal signal sequences and a significant number had previously demonstrated or possible novel roles in symbiosis, including bacterial cell surface modification, adhesins, proteins classified as MAMPs (microbe-associated molecular patterns), such as flagellin and EF-Tu, and several normally cytoplasmic proteins as Ndk and glycolytic enzymes, which are known to have extracellular "moonlighting" roles in bacteria that interact with eukaryotic cells. It is noteworthy that the transmembrane ß (1,2) glucan biosynthesis protein NdvB, an essential symbiotic protein in rhizobia, was found in the R. etli naringenin-induced exoproteome. In addition, potential binding sites for two nod-gene transcriptional regulators (NodD) occurred somewhat more frequently in the promoters of genes encoding naringenin-induced exoproteins in comparison to those ofexoproteins found in the control condition.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Services > Core Facility Massenspektrometrie- und Proteomics-Labor
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Protein- und Zellbiologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)

UniBE Contributor:

Meneses Moreno, Niurka, Buchs, Natasha, Heller, Manfred


600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology








Marla Rittiner

Date Deposited:

08 Mar 2018 13:45

Last Modified:

05 Dec 2022 15:09

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

Naringenin Nitrogen Fixation Proteomics Rhizobium




Actions (login required)

Edit item Edit item
Provide Feedback