Pooling of interdigital swab samples for PCR detection of virulent Dichelobacter nodosus.

Greber, Deborah; Locher, Iwan; Kuhnert, Peter; Butty, Marc André; Holdener, Kerstin; Frey, Joachim; Schüpbach, Gertraud; Steiner, Adrian (2018). Pooling of interdigital swab samples for PCR detection of virulent Dichelobacter nodosus. Journal of veterinary diagnostic investigation, 30(2), pp. 205-210. American Association of Veterinary Laboratory Diagnosticians AAVLD 10.1177/1040638717733508

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Virulent ovine foot rot is a contagious foot disease. Given the development and validation of a real-time PCR to detect Dichelobacter nodosus isolates that contain the virulence-associated protease genes aprV2 and aprB2, the diagnosis of foot rot has made considerable progress. We evaluated pooling methods to reduce the number of samples during a foot rot control program. Samples of individual feet were compared to a 4-feet sample of the same sheep. All further analyses based on 4-feet samples (pools-of-5 and pools-of-10 4-feet samples) were compared to samples of individual sheep, and a risk-based herd sampling was evaluated and compared to the whole flock. The sensitivity and specificity of the 4-feet samples for detection of aprV2-positive strains was 93.8% (CI: 87.6-97.5%) and 98.3% (CI: 96.5-99.3%), respectively. The sensitivity and specificity of the pools-of-10 was 86.7% (CI: 78.4-92.7%) and 100.0% (CI: 97.4-100%), respectively. Pools-of-5 were not significantly more sensitive than pools-of-10. The pooling of 4 individual foot samples into one 4-feet sample is an adequate method to reduce the number of samples of individual sheep. The sensitivity of pools-of-5 and pools-of-10 is too imprecise for a control program. Risk-based sampling allowed for a substantial reduction of samples to be tested, had a sensitivity of 95.8% (CI: 78.9-99.9%) and specificity of 100.0% (CI: 88.1-100.0%) when determining the foot rot flock status, and represents an adequate methodology to predict within-flock freedom from infection.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Veterinary Public Health / Herd Health Management
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Clinic for Ruminants
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Public Health Institute
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Greber, Deborah; Locher, Iwan; Kuhnert, Peter; Butty, Marc André; Frey, Joachim; Schüpbach, Gertraud and Steiner, Adrian

Subjects:

600 Technology > 630 Agriculture

ISSN:

1040-6387

Publisher:

American Association of Veterinary Laboratory Diagnosticians AAVLD

Language:

English

Submitter:

Susanne Agnes Lerch

Date Deposited:

16 Apr 2018 14:18

Last Modified:

09 Nov 2018 09:48

Publisher DOI:

10.1177/1040638717733508

PubMed ID:

29105601

Uncontrolled Keywords:

Dichelobacter nodosus aprV2 foot rot pooling real-time PCR sheep

BORIS DOI:

10.7892/boris.110158

URI:

https://boris.unibe.ch/id/eprint/110158

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