Nlrp6- and ASC-Dependent Inflammasomes Do Not Shape the Commensal Gut Microbiota Composition.

Mamantopoulos, Michail; Ronchi, Francesca; Van Hauwermeiren, Filip; Vieira-Silva, Sara; Yilmaz, Bahtiyar; Martens, Liesbet; Saeys, Yvan; Drexler, Stefan K; Yazdi, Amir S; Raes, Jeroen; Lamkanfi, Mohamed; McCoy, Kathleen; Wullaert, Andy (2017). Nlrp6- and ASC-Dependent Inflammasomes Do Not Shape the Commensal Gut Microbiota Composition. Immunity, 47(2), 339-348.e4. Cell Press 10.1016/j.immuni.2017.07.011

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The gut microbiota regulate susceptibility to multiple human diseases. The Nlrp6-ASC inflammasome is widely regarded as a hallmark host innate immune axis that shapes the gut microbiota composition. This notion stems from studies reporting dysbiosis in mice lacking these inflammasome components when compared with non-littermate wild-type animals. Here, we describe microbial analyses in inflammasome-deficient mice while minimizing non-genetic confounders using littermate-controlled Nlrp6-deficient mice and ex-germ-free littermate-controlled ASC-deficient mice that were all allowed to shape their gut microbiota naturally after birth. Careful microbial phylogenetic analyses of these cohorts failed to reveal regulation of the gut microbiota composition by the Nlrp6- and ASC-dependent inflammasomes. Our results obtained in two geographically separated animal facilities dismiss a generalizable impact of Nlrp6- and ASC-dependent inflammasomes on the composition of the commensal gut microbiota and highlight the necessity for littermate-controlled experimental design in assessing the influence of host immunity on gut microbial ecology.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Gastroenterology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Gastroenterologie / Mukosale Immunologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Gastroenterologie / Mukosale Immunologie

UniBE Contributor:

Ronchi, Francesca and McCoy, Kathleen

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1074-7613

Publisher:

Cell Press

Language:

English

Submitter:

Thi Thao Anh Pham

Date Deposited:

01 Feb 2018 10:19

Last Modified:

29 Oct 2019 03:56

Publisher DOI:

10.1016/j.immuni.2017.07.011

PubMed ID:

28801232

Uncontrolled Keywords:

16S sequencing ASC DSS colitis Nlrp6 caspase-1 dysbiosis gut microbiota inflammasomes innate immunity interleukin

BORIS DOI:

10.7892/boris.110305

URI:

https://boris.unibe.ch/id/eprint/110305

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