Gut Microbiome Standardization in Control and Experimental Mice.

McCoy, Kathy D; Geuking, Markus B; Ronchi, Francesca (2017). Gut Microbiome Standardization in Control and Experimental Mice. Current protocols in immunology, 117, 23.1.1-23.1.13. 10.1002/cpim.25

[img] Text
im2301.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (343kB) | Request a copy

Mouse models are used extensively to study human health and to investigate the mechanisms underlying human disease. In the past, most animal studies were performed without taking into consideration the impact of the microbiota. However, the microbiota that colonizes all body surfaces, including the gastrointestinal tract, respiratory tract, genitourinary tract, and skin, heavily impacts nearly every aspect of host physiology. When performing studies utilizing mouse models it is critical to understand that the microbiome is heavily impacted by environmental factors, including (but not limited to) food, bedding, caging, and temperature. In addition, stochastic changes in the microbiota can occur over time that also play a role in shaping microbial composition. These factors lead to massive variability in the composition of the microbiota between animal facilities and research institutions, and even within a single facility. Lack of experimental reproducibility between research groups has highlighted the necessity for rigorously controlled experimental designs in order to standardize the microbiota between control and experimental animals. Well controlled experiments are mandatory in order to reduce variability and allow correct interpretation of experimental results, not just of host-microbiome studies but of all mouse models of human disease. The protocols presented are aimed to design experiments that control the microbiota composition between different genetic strains of experimental mice within an animal unit. © 2017 by John Wiley & Sons, Inc.

Item Type:

Journal Article (Further Contribution)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Gastroenterology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Gastroenterologie / Mukosale Immunologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Gastroenterologie / Mukosale Immunologie

UniBE Contributor:

Ronchi, Francesca

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1934-368X

Language:

English

Submitter:

Thi Thao Anh Pham

Date Deposited:

06 Feb 2018 11:25

Last Modified:

08 Feb 2018 08:36

Publisher DOI:

10.1002/cpim.25

PubMed ID:

28369684

Uncontrolled Keywords:

co-housing fecal transplants germ-free litter swaps littermate controls microbiota standardization

BORIS DOI:

10.7892/boris.110307

URI:

https://boris.unibe.ch/id/eprint/110307

Actions (login required)

Edit item Edit item
Provide Feedback