Drought induced leaf protein alterations in sensitive and tolerant wheat varieties

Demirevska, K.; Simova-Stoilova, Lyudmila; Vassileva, Valya; Vaseva, Irina; Grigorova, B.; Feller, Urs (2008). Drought induced leaf protein alterations in sensitive and tolerant wheat varieties. General and Applied Plant Physiology, 34(1-2), pp. 79-102. Acad. M. Popov, Institute of Plant Physiology

Text 2008_GenApplPlantPhys_SI_34_79.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (964kB) | Request a copy

Wheat plants with a fully developed first leaf were subjected to severe but recoverable water stress. Leaves from drought tolerant (Katya and Zlatitza) and drought sensitive (Sadovo and Miziya) varieties in control, drought and recovery conditions were used for the experiments. The physiological response of drought tolerant varieties did not differ from the one of drought sensitive varieties at early seedling stage under these conditions. A comparative study of the ultrathin sections by transmission electron microscopy from control and drought stressed plants revealed prominent changes in mitochondrion fine structures. The relative cell area occupied by mitochondria was reduced in the drought sensitive varieties. An increased quantity of abscisic acid (ABA) was detected in drought stressed wheat plants. The drought sensitive varieties (Miziya and Sadovo) possessed higher azocaseinolytic activity. An immunoblotting analysis was performed for some specificity detection of protein response under drought conditions of Rubisco, Rubisco activase (RA), Rubisco binding protein (RBP), dehydrins (DHN), some heat shock proteins (HSP) and ATP dependent calpain protease (Clp) proteins. The obtained results showed that the drought tolerant varieties Katya and Zlatitza had higher levels of these proteins, especially RBP and Clp proteases. Our attention was focused on the coordinated response of Rubisco, RA, RBP, DHNs, HSPs and Clp proteases in stress conditions, such as drought. The biochemical response diversity in susceptible and tolerant wheat varieties is discussed.

Interest & Impact

Downloads

1 since deposited on 07 Feb 2018
0 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item