The association between progestins, nuclear receptors expression and inflammation in endometrial stromal cells from women with endometriosis.

Grandi, Giovanni; Mueller, Michael; Bersinger, Nick A.; Facchinetti, Fabio; Mc Kinnon, Brett (2017). The association between progestins, nuclear receptors expression and inflammation in endometrial stromal cells from women with endometriosis. Gynecological endocrinology, 33(9), pp. 712-715. Taylor & Francis 10.1080/09513590.2017.1314458

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Endometriosis is an inflammatory disease and nuclear receptors play a crucial role in mediating the inflammatory response. In endometrial stromal cells (ESC), nuclear receptors expression can be influenced by the local environment. Progestins are first-line, on-label treatments of endometriosis that may have direct effects on endometriotic lesions through these nuclear receptors. Therefore, we investigated whether there was an association between nuclear receptors expression and the influence of progestins on inflammatory cytokines production in a preliminary, in vitro study with primary cultures. ESC from endometrial biopsies of six subjects with histologically confirmed endometriosis were treated for 6 h with medium alone or with TNF-α (10 or 100 ng/ml) in the presence of dienogest (DNG), medroxyprogesterone acetate (MPA) and norethisterone acetate (NETA) 10-5 M. The progestin-mediated change in IL6, IL8 and MCP-1 mRNA transcription was measured, as was the PRA, PRB, GR, AR and MCR protein expression. The change (medium versus TNF-α 10 ng/ml and medium versus TNF-α 100 ng/ml) in IL6 mRNA transcription was positively associated with the change in PRB, but not PRA with both DNG and NETA treatment. The change in IL8 mRNA was negatively associated with AR expression in the presence of NETA. The change in MCP-1 mRNA expression was positively associated with GR expression and negatively associated with MCR after MPA treatment. The associations between the change in cytokines mRNA expression and nuclear receptors protein expression in response to progestins activity may indirectly suggest different activities of these compounds at a local level worthy of further investigations.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Endometriose und gynäkologische Onkologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Endometriose und gynäkologische Onkologie

UniBE Contributor:

Mueller, Michael; Bersinger, Nick A. and Mc Kinnon, Brett

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0951-3590

Publisher:

Taylor & Francis

Language:

English

Submitter:

Monika Zehr

Date Deposited:

22 Feb 2018 16:50

Last Modified:

22 Feb 2018 16:50

Publisher DOI:

10.1080/09513590.2017.1314458

PubMed ID:

28412861

Uncontrolled Keywords:

Androgen receptor dienogest medroxyprogesterone acetate norethisterone acetate progesterone receptors

URI:

https://boris.unibe.ch/id/eprint/111018

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