Injectable platelet rich fibrin (i-PRF): opportunities in regenerative dentistry?

Miron, Richard John; Kobayashi, Masako; Hernandez, Maria; Kandalam, Umadevi; Zhang, Yufeng; Ghanaati, Shahram; Choukroun, Joseph (2017). Injectable platelet rich fibrin (i-PRF): opportunities in regenerative dentistry? Clinical oral investigations, 21(8), pp. 2619-2627. Springer 10.1007/s00784-017-2063-9

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OBJECTIVES Platelet rich plasma (PRP) has been utilized in regenerative dentistry as a supra-physiological concentrate of autologous growth factors capable of stimulating tissue regeneration. Despite this, concerns have been expressed regarding the use of anti-coagulants, agents known to inhibit wound healing. In this study, a liquid formulation of platelet rich fibrin (PRF) termed injectable-PRF (i-PRF) without the use of anti-coagulants was investigated. MATERIALS AND METHODS Standard PRP and i-PRF (centrifuged at 700 rpm (60G) for 3 min) were compared for growth factor release up to 10 days (8 donor samples). Furthermore, fibroblast biocompatibility at 24 h (live/dead assay); migration at 24 h; proliferation at 1, 3, and 5 days, and expression of PDGF, TGF-β, and collagen1 at 3 and 7 days were investigated. RESULTS Growth factor release demonstrated that in general PRP had higher early release of growth factors whereas i-PRF showed significantly higher levels of total long-term release of PDGF-AA, PDGF-AB, EGF, and IGF-1 after 10 days. PRP showed higher levels of TGF-β1 and VEGF at 10 days. While both formulations exhibited high biocompatibility and higher fibroblast migration and proliferation when compared to control tissue-culture plastic, i-PRF induced significantly highest migration whereas PRP demonstrated significantly highest cellular proliferation. Furthermore, i-PRF showed significantly highest mRNA levels of TGF-β at 7 days, PDGF at 3 days, and collagen1 expression at both 3 and 7 days when compared to PRP. CONCLUSIONS i-PRF demonstrated the ability to release higher concentrations of various growth factors and induced higher fibroblast migration and expression of PDGF, TGF-β, and collagen1. Future animal research is now necessary to further validate the use of i-PRF as a bioactive agent capable of stimulating tissue regeneration. CLINICAL RELEVANCE The findings from the present study demonstrate that a potent formulation of liquid platelet concentrates could be obtained without use of anti-coagulants.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery

UniBE Contributor:

Miron, Richard John and Kobayashi, Masako

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1432-6981

Publisher:

Springer

Language:

English

Submitter:

Caroline Dominique Zürcher

Date Deposited:

12 Mar 2018 15:25

Last Modified:

29 Oct 2019 19:13

Publisher DOI:

10.1007/s00784-017-2063-9

PubMed ID:

28154995

Uncontrolled Keywords:

Blood platelets Fibrin Fibroblasts Platelet rich fibrin Regeneration Wound healing

BORIS DOI:

10.7892/boris.111127

URI:

https://boris.unibe.ch/id/eprint/111127

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