Fujikawa, Yuuta; Roma, Leticia P; Sobotta, Mirko C; Rose, Adam J; Diaz, Mauricio Berriel; Locatelli, Giuseppe; Breckwoldt, Michael O; Misgeld, Thomas; Kerschensteiner, Martin; Herzig, Stephan; Müller-Decker, Karin; Dick, Tobias P (2016). Mouse redox histology using genetically encoded probes. Science signaling, 9(419), rs1. American Association for the Advancement of Science 10.1126/scisignal.aad3895
Full text not available from this repository. (Request a copy)Mapping the in vivo distribution of endogenous oxidants in animal tissues is of substantial biomedical interest. Numerous health-related factors, including diet, physical activity, infection, aging, toxins, or pharmacological intervention, may cause redox changes. Tools are needed to pinpoint redox state changes to particular organs, tissues, cell types, and subcellular organelles. We describe a procedure that preserves the in vivo redox state of genetically encoded redox biosensors within histological tissue sections, thus providing "redox maps" for any tissue and comparison of interest. We demonstrate the utility of the technique by visualizing endogenous redox differences and changes in the context of tumor growth, inflammation, embryonic development, and nutrient starvation.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute |
UniBE Contributor: |
Locatelli, Giuseppe |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1937-9145 |
Publisher: |
American Association for the Advancement of Science |
Language: |
English |
Submitter: |
Ursula Zingg-Zünd |
Date Deposited: |
22 Mar 2018 16:29 |
Last Modified: |
05 Dec 2022 15:10 |
Publisher DOI: |
10.1126/scisignal.aad3895 |
PubMed ID: |
26980443 |
URI: |
https://boris.unibe.ch/id/eprint/111246 |
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