The choroid plexus is a key cerebral invasion route for T cells after stroke.

Llovera, Gemma; Benakis, Corinne; Enzmann, Gaby; Cai, Ruiyao; Arzberger, Thomas; Ghasemigharagoz, Alireza; Mao, Xiang; Malik, Rainer; Lazarevic, Ivana; Liebscher, Sabine; Ertürk, Ali; Meissner, Lilja; Vivien, Denis; Haffner, Christof; Plesnila, Nikolaus; Montaner, Joan; Engelhardt, Britta; Liesz, Arthur (2017). The choroid plexus is a key cerebral invasion route for T cells after stroke. Acta neuropathologica, 134(6), pp. 851-868. Springer 10.1007/s00401-017-1758-y

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Neuroinflammation contributes substantially to stroke pathophysiology. Cerebral invasion of peripheral leukocytes-particularly T cells-has been shown to be a key event promoting inflammatory tissue damage after stroke. While previous research has focused on the vascular invasion of T cells into the ischemic brain, the choroid plexus (ChP) as an alternative cerebral T-cell invasion route after stroke has not been investigated. We here report specific accumulation of T cells in the peri-infarct cortex and detection of T cells as the predominant population in the ipsilateral ChP in mice as well as in human post-stroke autopsy samples. T-cell migration from the ChP to the peri-infarct cortex was confirmed by in vivo cell tracking of photoactivated T cells. In turn, significantly less T cells invaded the ischemic brain after photothrombotic lesion of the ipsilateral ChP and in a stroke model encompassing ChP ischemia. We detected a gradient of CCR2 ligands as the potential driving force and characterized the neuroanatomical pathway for the intracerebral migration. In summary, our study demonstrates that the ChP is a key invasion route for post-stroke cerebral T-cell invasion and describes a CCR2-ligand gradient between cortex and ChP as the potential driving mechanism for this invasion route.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute

UniBE Contributor:

Enzmann, Gaby; Lazarevic, Ivana and Engelhardt, Britta

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0001-6322

Publisher:

Springer

Language:

English

Submitter:

Ursula Zingg-Zünd

Date Deposited:

22 Mar 2018 15:36

Last Modified:

22 Mar 2018 16:11

Publisher DOI:

10.1007/s00401-017-1758-y

PubMed ID:

28762187

BORIS DOI:

10.7892/boris.111257

URI:

https://boris.unibe.ch/id/eprint/111257

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