Molecular model of the mitochondrial genome segregation machinery in Trypanosoma brucei

Hoffmann, Anneliese; Käser, Sandro; Jakob, Martin; Amodeo, Simona; Peitsch, Camille Françoise; Týč, Jiří; Vaughan, Sue; Zuber, Benoît; Schneider, André; Ochsenreiter, Torsten (2018). Molecular model of the mitochondrial genome segregation machinery in Trypanosoma brucei. Proceedings of the National Academy of Sciences of the United States of America - PNAS, 115(8), E1809-E1818. National Academy of Sciences NAS 10.1073/pnas.1716582115

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In almost all eukaryotes mitochondria maintain their own genome. Despite the discovery more than 50 years ago still very little is known about how the genome is properly segregated during cell division. The protozoan parasite Trypanosoma brucei contains a single mitochondrion with a singular genome the kinetoplast DNA (kDNA). Electron microscopy studies revealed the tripartite attachment complex (TAC) to physically connect the kDNA to the basal body of the flagellum and to ensure proper segregation of the mitochondrial genome via the basal bodies movement, during cell cycle. Using super-resolution microscopy we precisely localize each of the currently known unique TAC components. We demonstrate that the TAC is assembled in a hierarchical order from the base of the flagellum towards the mitochondrial genome and that the assembly is not dependent on the kDNA itself. Based on biochemical analysis the TAC consists of several non-overlapping subcomplexes suggesting an overall size of the TAC exceeding 2.8 mDa. We furthermore demonstrate that the TAC has an impact on mitochondrial organelle positioning however is not required for proper organelle biogenesis or segregation.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
08 Faculty of Science > Departement of Chemistry and Biochemistry
08 Faculty of Science > Department of Biology > Institute of Cell Biology
09 Interdisciplinary Units > Microscopy Imaging Center MIC

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Hoffmann, Anneliese; Käser, Sandro; Jakob, Martin; Amodeo, Simona; Peitsch, Camille Françoise; Zuber, Benoît; Schneider, André and Ochsenreiter, Torsten

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

0027-8424

Publisher:

National Academy of Sciences NAS

Language:

English

Submitter:

Christina Schüpbach

Date Deposited:

08 Feb 2018 15:21

Last Modified:

17 Jan 2019 10:03

Publisher DOI:

10.1073/pnas.1716582115

BORIS DOI:

10.7892/boris.111274

URI:

https://boris.unibe.ch/id/eprint/111274

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