A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity.

Sachs, Norman; de Ligt, Joep; Kopper, Oded; Gogola, Ewa; Bounova, Gergana; Weeber, Fleur; Balgobind, Anjali Vanita; Wind, Karin; Gracanin, Ana; Begthel, Harry; Korving, Jeroen; van Boxtel, Ruben; Duarte, Alexandra Alves; Lelieveld, Daphne; van Hoeck, Arne; Ernst, Robert Frans; Blokzijl, Francis; Nijman, Isaac Johannes; Hoogstraat, Marlous; van de Ven, Marieke; ... (2018). A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity. Cell, 172(1-2), pp. 373-386. Cell Press 10.1016/j.cell.2017.11.010

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Breast cancer (BC) comprises multiple distinct subtypes that differ genetically, pathologically, and clinically. Here, we describe a robust protocol for long-term culturing of human mammary epithelial organoids. Using this protocol, >100 primary and metastatic BC organoid lines were generated, broadly recapitulating the diversity of the disease. BC organoid morphologies typically matched the histopathology, hormone receptor status, and HER2 status of the original tumor. DNA copy number variations as well as sequence changes were consistent within tumor-organoid pairs and largely retained even after extended passaging. BC organoids furthermore populated all major gene-expression-based classification groups and allowed in vitro drug screens that were consistent with in vivo xeno-transplantations and patient response. This study describes a representative collection of well-characterized BC organoids available for cancer research and drug development, as well as a strategy to assess in vitro drug response in a personalized fashion.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Rottenberg, Sven

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

0092-8674

Publisher:

Cell Press

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

18 Apr 2018 16:26

Last Modified:

18 Apr 2018 16:27

Publisher DOI:

10.1016/j.cell.2017.11.010

PubMed ID:

29224780

Uncontrolled Keywords:

basal biobank breast cancer luminal organoids precision medicine triple negative

BORIS DOI:

10.7892/boris.112034

URI:

https://boris.unibe.ch/id/eprint/112034

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