Comparison among gold standard techniques used for the validation of methods for occlusal caries detection

Rodrigues, Jonas A; Neuhaus, Klaus W; Diniz, Michele B; Hug, Isabel; Stich, Herman; Karlsson, Lena; Lussi, Adrian (2012). Comparison among gold standard techniques used for the validation of methods for occlusal caries detection. Microscopy research and technique, 75(5), pp. 605-8. New York, N.Y.: Wiley-Liss 10.1002/jemt.21097

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The aim of this in vitro study was to assess the agreement among four techniques used as gold standard for the validation of methods for occlusal caries detection. Sixty-five human permanent molars were selected and one site in each occlusal surface was chosen as the test site. The teeth were cut and prepared according to each technique: stereomicroscopy without coloring (1), dye enhancement with rhodamine B (2) and fuchsine/acetic light green (3), and semi-quantitative microradiography (4). Digital photographs from each prepared tooth were assessed by three examiners for caries extension. Weighted kappa, as well as Friedman's test with multiple comparisons, was performed to compare all techniques and verify statistical significant differences. Results: kappa values varied from 0.62 to 0.78, the latter being found by both dye enhancement methods. Friedman's test showed statistical significant difference (P < 0.001) and multiple comparison identified these differences among all techniques, except between both dye enhancement methods (rhodamine B and fuchsine/acetic light green). Cross-tabulation showed that the stereomicroscopy overscored the lesions. Both dye enhancement methods showed a good agreement, while stereomicroscopy overscored the lesions. Furthermore, the outcome of caries diagnostic tests may be influenced by the validation method applied. Dye enhancement methods seem to be reliable as gold standard methods.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry

UniBE Contributor:

Neuhaus, Klaus; Hug, Isabelle; Stich, Hermann and Lussi, Adrian








Eveline Carmen Schuler

Date Deposited:

04 Oct 2013 14:30

Last Modified:

25 Jan 2017 12:16

Publisher DOI:


PubMed ID:


Web of Science ID:


URI: (FactScience: 217207)

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