Glycan-binding specificities of Streptococcus mutans and Streptococcus sobrinus lectin-like adhesins

Schüler, Viviane; Lussi, Adrian; Kage, Andreas; Seemann, Rainer (2012). Glycan-binding specificities of Streptococcus mutans and Streptococcus sobrinus lectin-like adhesins. Clinical oral investigations, 16(3), pp. 789-96. Berlin: Springer-Verlag 10.1007/s00784-011-0568-1

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Since the adhesion of bacteria to the tooth surface is a prerequisite for dental plaque and subsequent caries development, a promising caries preventive strategy could be to block the lectin-glycan-mediated adherence of cariogenic bacteria. The aim of the study was to evaluate potential differences in glycan-binding specificities of two Streptococcus mutans strains (DSM 20523 and DSM 6178) and Streptococcus sobrinus (DSM 20381). A competitive enzyme-linked lectin-binding assay was used to identify the binding specificities of isolated bacterial surface lectins. Blotting of the microbial proteins on neoglycoprotein-coated PVP membranes enabled a qualitative protein analysis of all specific bacterial lectins. Different glycan-binding sites could be identified for the S. mutans strains in comparison to S. sobrinus. An earlier reported glycan-binding specificity for terminal galactose residues could be confirmed for the S. mutans strains. For the S. sobrinus strain, more than one glycan-binding specificity could be found (oligomannose and terminal sialyl residues). Each of the tested strains showed more than one surface lectin responsible for the specific lectin-binding with varying molecular weight (S. mutans, 90/155 kDa and S. sobrinus, 35/45 kDa). The established experimental setup could be used as future standard procedure for the identification of bacterial lectin-derived binding specificities. The findings from this study might serve as basis for the design of an individual 'glycan cocktail' for the competitive inhibition of lectin-mediated adhesion of mutans streptococci to oral surfaces.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry

UniBE Contributor:

Lussi, Adrian and Seemann, Rainer

ISSN:

1432-6981

Publisher:

Springer-Verlag

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

04 Oct 2013 14:30

Last Modified:

26 Jun 2018 14:34

Publisher DOI:

10.1007/s00784-011-0568-1

PubMed ID:

21681389

Web of Science ID:

000304173900014

BORIS DOI:

10.7892/boris.11228

URI:

https://boris.unibe.ch/id/eprint/11228 (FactScience: 217258)

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