Comparison of three strip-type tests and two laboratory methods for salivary buffering analysis

Cheaib, Zeinab; Ganss, Carolina; Lamanda, Andreas; Turgut, Melek Dilek; Lussi, Adrian (2012). Comparison of three strip-type tests and two laboratory methods for salivary buffering analysis. Odontology, 100(1), pp. 67-75. Tokyo: Springer Japan 10.1007/s10266-011-0018-1

[img] Text
11229.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (243kB) | Request a copy
[img]
Preview
Text
10266_2011_Article_18.pdf - Other
Available under License Publisher holds Copyright.

Download (243kB) | Preview

This study evaluated the correlation between three strip-type, colorimetric tests and two laboratory methods with respect to the analysis of salivary buffering. The strip-type tests were saliva-check buffer, Dentobuff strip and CRT(®) Buffer test. The laboratory methods included Ericsson's laboratory method and a monotone acid/base titration to create a reference scale for the salivary titratable acidity. Additionally, defined buffer solutions were prepared and tested to simulate the carbonate, phosphate and protein buffer systems of saliva. The correlation between the methods was analysed by the Spearman's rank test. Disagreement was detected between buffering capacity values obtained with three strip-type tests that was more pronounced in case of saliva samples with medium and low buffering capacities. All strip-type tests were able to assign the hydrogencarbonate, di-hydrogenphosphate and 0.1% protein buffer solutions to the correct buffer categories. However, at 0.6% total protein concentrations, none of the test systems worked accurately. Improvements are necessary for strip-type tests because of certain disagreement with the Ericsson's laboratory method and dependence on the protein content of saliva.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry

UniBE Contributor:

Cheaib, Zeinab and Lussi, Adrian

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1618-1247

Publisher:

Springer Japan

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

04 Oct 2013 14:30

Last Modified:

26 Jun 2018 13:28

Publisher DOI:

10.1007/s10266-011-0018-1

PubMed ID:

21556727

Web of Science ID:

000304421100010

BORIS DOI:

10.7892/boris.11229

URI:

https://boris.unibe.ch/id/eprint/11229 (FactScience: 217259)

Actions (login required)

Edit item Edit item
Provide Feedback