Enamel matrix protein adsorption to root surfaces in the presence or absence of human blood

Miron, Richard; Bosshardt, Dieter; Laugisch, Oliver; Katsaros, Christos; Buser, Daniel; Sculean, Anton (2012). Enamel matrix protein adsorption to root surfaces in the presence or absence of human blood. Journal of periodontology, 83(7), pp. 885-892. Chicago, Ill.: American Academy of Periodontology 10.1902/jop.2011.110404

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Background: The clinical use of an enamel matrix derivative (EMD) has been shown to promote formation of new cementum, periodontal ligament (PDL), and bone and to significantly enhance the clinical outcomes after regenerative periodontal surgery. It is currently unknown to what extent the bleeding during periodontal surgery may compete with EMD adsorption to root surfaces. The aim of this study is to evaluate the effect of blood interactions on EMD adsorption to root surfaces mimicking various clinical settings and to test their ability to influence human PDL cell attachment and proliferation. Methods: Teeth extracted for orthodontic reasons were subjected to ex vivo scaling and root planing and treated with 24% EDTA, EMD, and/or human blood in six clinically related settings to determine the ability of EMD to adsorb to root surfaces. Surfaces were analyzed for protein adsorption via scanning electron microscopy and immunohistochemical staining with an anti-EMD antibody. Primary human PDL cells were seeded on root surfaces and quantified for cell attachment and cell proliferation. Results: Plasma proteins from blood samples altered the ability of EMD to adsorb to root surfaces on human teeth. Samples coated with EMD lacking blood demonstrated a consistent even layer of EMD adsorption to the root surface. In vitro experiments with PDL cells demonstrated improved cell attachment and proliferation in all samples coated with EMD (irrespective of EDTA) when compared to samples containing human blood. Conclusion: Based on these findings, it is advised to minimize blood interactions during periodontal surgeries to allow better adsorption of EMD to root surfaces.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > Department of Oral Surgery and Stomatology
04 Faculty of Medicine > School of Dental Medicine > Department of Orthodontics

UniBE Contributor:

Miron, Richard; Bosshardt, Dieter; Laugisch, Oliver; Katsaros, Christos; Buser, Daniel and Sculean, Anton

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0022-3492

Publisher:

American Academy of Periodontology

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

04 Oct 2013 14:30

Last Modified:

25 Jan 2017 12:16

Publisher DOI:

10.1902/jop.2011.110404

PubMed ID:

22122520

Web of Science ID:

000306535700010

BORIS DOI:

10.7892/boris.11263

URI:

https://boris.unibe.ch/id/eprint/11263 (FactScience: 217338)

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