Dicer-like Enzymes with Sequence Cleavage Preferences

Nowacki, Mariusz; Höhener, Cristina; Hug, Iris Sandra (2018). Dicer-like Enzymes with Sequence Cleavage Preferences. Cell, 173(1), 234-247.e7. Cell Press 10.1016/j.cell.2018.02.029

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Dicer proteins are known to produce small RNAs (sRNAs) from long double-stranded RNA (dsRNA) templates. These sRNAs are bound by Argonaute proteins, which select the guide strand, often with a 5′ end sequence bias. However, Dicer proteins have never been shown to have sequence cleavage preferences. In Paramecium development, two classes of sRNAs that are required for DNA elimination are produced by three Dicer-like enzymes: Dcl2, Dcl3, and Dcl5. Through in vitro cleavage assays, we demonstrate that Dcl2 has a strict size preference for 25 nt and a sequence preference for 5′ U and 5′ AGA, while Dcl3 has a sequence preference for 5′ UNG. Dcl5, however, has cleavage preferences for 5′ UAG and 3′ CUAC/UN, which leads to the production of RNAs precisely matching short excised DNA elements with corresponding end base preferences. Thus, we characterize three Dicer-like enzymes that are involved in Paramecium development and propose a biological role for their sequence-biased cleavage products.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology > Ciliate
08 Faculty of Science > Department of Biology > Institute of Cell Biology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Nowacki, Mariusz; Höhener, Cristina and Hug, Iris Sandra

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0092-8674

Publisher:

Cell Press

Funders:

[18] European Research Council
[4] Swiss National Science Foundation
[108] NCCR RNA & Disease

Language:

English

Submitter:

Mariusz Nowacki

Date Deposited:

08 May 2018 14:44

Last Modified:

08 May 2018 14:44

Publisher DOI:

10.1016/j.cell.2018.02.029

PubMed ID:

29551264

BORIS DOI:

10.7892/boris.113186

URI:

https://boris.unibe.ch/id/eprint/113186

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