Stauffacher, Simone; Lussi, Adrian; Nietzsche, Sandor; Neuhaus, Klaus; Eick, Sigrun (2017). Bacterial invasion into radicular dentine-an in vitro study. Clinical oral investigations, 21(5), pp. 1743-1752. Springer-Verlag 10.1007/s00784-016-1960-7
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OBJECTIVES
We wanted to investigate differences in invasiveness into radicular dentinal tubules by monocultured and co-cultured bacteria frequently found in infected root canals.
METHODS
Fifty-one human roots were incubated for 8 weeks with monocultured Streptococcus gordonii ATCC 10558, Streptococcus sanguinis ATCC 10556, and with five capnophiles/anaerobes as well as with capnophiles/anaerobes co-cultured with a streptococcal species. Thereafter, bacterial samples were cultured from the inner, middle, and outer third of the root dentine of longitudinally broken teeth (n = 5). In addition, scanning electron microscopy (SEM) images were obtained.
RESULTS
Single gram-positive species were able to penetrate into the middle and outer third of the root dentine. Fusobacterium nucleatum ATCC 25586 was not found in any of the dentine specimens. Prevotella intermedia ATCC 25611 and Porphyromonas gingivalis ATCC 33277 were found in the inner and middle third. The bacterial load of streptococci was higher in all thirds in co-cultures compared to single infections. In co-cultures with streptococci, Actinomyces oris ATCC 43146 was found in the outer third in 9/10 samples, whereas P. intermedia ATCC 25611 was not detectable inside dentine. Co-culture with S. sanguinis ATCC 10556 enabled F. nucleatum ATCC 25586 to invade dentine; SEM images showed that F. nucleatum ATCC 25586 had a swollen shape.
CONCLUSIONS
Invasiveness of bacteria into dentinal tubules is species-specific and may change depending on culturing as a single species or co-culturing with other bacteria.
CLINICAL RELEVANCE
Oral streptococci may promote or inhibit invasion of capnophiles/anaerobes into radicular dentine.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Periodontics Research 04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry |
UniBE Contributor: |
Stauffacher, Simone, Lussi, Adrian, Neuhaus, Klaus, Eick, Sigrun |
Subjects: |
600 Technology > 610 Medicine & health 500 Science > 570 Life sciences; biology |
ISSN: |
1432-6981 |
Publisher: |
Springer-Verlag |
Language: |
English |
Submitter: |
Klaus Neuhaus |
Date Deposited: |
12 Jul 2018 16:35 |
Last Modified: |
05 Dec 2022 15:12 |
Publisher DOI: |
10.1007/s00784-016-1960-7 |
PubMed ID: |
27722787 |
Uncontrolled Keywords: |
Bacterial invasion, Co-culture, Dentinal tubules, Monoculture, Root dentine, Scanning electron microscopy |
BORIS DOI: |
10.7892/boris.114373 |
URI: |
https://boris.unibe.ch/id/eprint/114373 |
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