Stage-specific expression of the proline-alanine transporter in the human pathogen Leishmania

Liburkin-Dan, T.; Schlisselberg, D.; Fischer-Weinberger, R.; Pescher, P.; Inbar, E.; Ephros, M.; Rentsch, Doris; Späth, G.F.; Zilberstein, D. (2018). Stage-specific expression of the proline-alanine transporter in the human pathogen Leishmania. Molecular and biochemical parasitology, 222, pp. 1-5. Elsevier 10.1016/j.molbiopara.2018.04.002

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Leishmania are obligatory intracellular parasites that cycle between the sand fly midgut (extracellular promastigotes) and mammalian macrophage phagolysosomes (intracellular amastigotes). They have developed mechanisms of adaptation to the distinct environments of host and vector that favor utilization of both proline and alanine. LdAAP24 is the L. donovani proline-alanine transporter. It is a member of Leishmania system A that translocates neutral amino acids. Since system A is promastigote-specific, we aimed to assess whether LdAAP24 is also expressed exclusively in promastigotes. Herein, we established that upon exposing L. donovani promastigotes to amastigote differentiation signal (pH 5.5 and 37 °C), parasites rapidly and completely degrade LdAAP24 protein in both axenic and in spleen-derived amastigotes. In contrast, LdAAP24 mRNA remained unchanged throughout differentiation. Addition of either MG132 or Bafilomycin A1 partially inhibited LdAAP24 protein degradation, indicating a role for both lysosome- and proteasome-mediated degradation. This work provides the first evidence for post-translational regulation of stage-specific expression of LdAAP24.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Molecular Plant Physiology
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Rentsch, Doris

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0166-6851

Publisher:

Elsevier

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

02 May 2018 11:02

Last Modified:

28 Oct 2019 10:54

Publisher DOI:

10.1016/j.molbiopara.2018.04.002

Uncontrolled Keywords:

Leishmania; amino acid transport; stage-specific expression; protein degradation; differentiation; signal

BORIS DOI:

10.7892/boris.114853

URI:

https://boris.unibe.ch/id/eprint/114853

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