Distribution and density of α– and β–adrenergic receptor binding sites in the bovine mammary gland

Hammon, Harald M.; Bruckmaier, Rupert M.; Honegger, Ulrich E.; Blum, Jürg W. (1994). Distribution and density of α– and β–adrenergic receptor binding sites in the bovine mammary gland. Journal of dairy research, 61(1), pp. 47-57. Cambridge University Press 10.1017/S0022029900028041

S0022029900028041.pdf - Published Version
Available under License Publisher holds Copyright.

Download (751kB) | Preview

Radioreceptor binding studies were designed to localize and determine the number of α and β-adrenergic receptors in the mammary gland of lactating cows. 3H-prazosin, 3H-rauwolscine and 3H-dihydroalprenolol were used for the regional characterization of α1, α2- and β-adrenergic receptors by competitive inhibition of binding of 3H-ligands with unlabelled adrenergic agonists and antagonists. The α1-, α2- and β2-adrenergic receptor subtypes could thus be demonstrated in the regions of the teats, large mammary ducts and parenchyma. Tissues of the teat wall, of the large mammary ducts above the gland cistern and of the mammary parenchyma were prepared to determine the density of α1, α2- and β-receptors by saturation binding assays using 3H-prazosin, 3H-rauwolscine and 3H-dihydroalprenolol respectively. Binding to high affinity sites was reversible within minutes and saturable. Equilibrium was reached within minutes. The number of α1-and α2-adrenergic receptors decreased from the teat to the mammary ducts to the parenchyma. Most of the α1- and α2-adrenergic receptors were found in the teat wall, whereas in the parenchyma α-adrenergic receptors were absent or barely detectable. The density of β-adrenergic receptors was similar in the teat wall and the large mammary ducts, but much lower in the parenchyma. Thus, α1, α2- and β-adrenergic receptors were found mainly in the milk purging system and hardly at all in mammary parenchyma. Inhibition of milk removal by α-adrenergic stimulation is possibly due to constriction of teat wall and to constriction of the mammary ducts, whereas enhanced milk flow after β-adrenergic stimulation is possibly due to relaxation not only of the teat sphincter and teat wall, but probably also of the large mammary ducts.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Pharmacology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Physiology

UniBE Contributor:

Bruckmaier, Rupert; Honegger, Ulrich and Blum, Jürg


600 Technology > 610 Medicine & health




Cambridge University Press




Marceline Brodmann

Date Deposited:

08 Jul 2020 14:25

Last Modified:

04 Aug 2020 18:00

Publisher DOI:






Actions (login required)

Edit item Edit item
Provide Feedback