Identification and characterization of two repetitive non-variable antigens from African trypanosomes which are recognized early during infection

Müller, N.; Hemphill, A.; Imboden, M.; Duvallet, G.; Dwinger, R. H.; Seebeck, T. (1992). Identification and characterization of two repetitive non-variable antigens from African trypanosomes which are recognized early during infection. Parasitology, 104(1), pp. 111-120. Cambridge University Press 10.1017/S0031182000060856

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The present paper describes two repetitive proteins representing common antigens of African trypanosomes which are non-variant and which are recognized early in infection by the host immune system. These antigens were identified by their ability to immunoreact with bovine serum taken during the early phase of a cyclic trypanosomal infection. Screening of a cDNA library from T. b. gambiense with such early infection serum identified a protein which contains a repetitive motif consisting of 68 amino acid repeat units (GM6). Immunofluorescence and immunogold electron microscopy revealed that GM6 is located on fibres which connect the microtubules of the membrane skeleton with the flagellum. A second repetitive antigen detected by this serum is MARP1 (microtubule-associated repetitive protein 1), a protein previously characterized in this laboratory as a high-molecular weight component of the membrane skeleton, which consists of more than 50 tandemly repeated, near-identical 38 amino acid repeat units. Beta-galactosidase fusion products of both proteins demonstrated a strong immunoreactivity with sera from T. b. brucei and T. congolense-infected cattle. The result from this preliminary immunological evaluation indicates a high immunodiagnostic sensititivy (90%) of the two recombinant antigens which make them interesting candidates for immunodiagnosis of trypanosomiasis in cattle.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Seebeck, Thomas

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0031-1820

Publisher:

Cambridge University Press

Language:

English

Submitter:

Marceline Brodmann

Date Deposited:

16 Jul 2020 09:53

Last Modified:

24 Jul 2020 18:55

Publisher DOI:

10.1017/S0031182000060856

PubMed ID:

1614728

BORIS DOI:

10.7892/boris.115442

URI:

https://boris.unibe.ch/id/eprint/115442

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