Characterization of a 14 kDa oocyst wall protein of Eimeria tenella and E. acervulina

Eschenbacher, K.-H.; Eggli, Peter; Wallach, M.; Braun, R. (1996). Characterization of a 14 kDa oocyst wall protein of Eimeria tenella and E. acervulina. Parasitology, 112(2), pp. 169-176. Cambridge University Press 10.1017/S0031182000084730

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We have extracted a protein of 14 kDa from purified oocyst walls of several Eimeria species. Polyclonal antibodies were raised in rats against the 14 kDa proteins of E. acervulina and E. tenella. On immunoblots these antisera reacted in a highly specific manner with the homologous 14 kDa antigens, but not with heterologous antigens. In addition, specific binding of the two antisera to oocyst wall fragments of E. acervulina and E. tenella was demonstrated by immunofluorescence. Partial amino-terminal sequences comprising 20 amino acid residues were obtained from the 14 kDa oocyst wall proteins of E. acervulina and E. tenella. They are characterized by an abundance of amino acids containing hydroxyl groups in their side chains (serine, tyrosine, threonine). Binding of the oocyst wall protein of E. tenella by peanut agglutinin indicates the presence of O-linked carbohydrates.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Eggli, Peter Slade

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0031-1820

Publisher:

Cambridge University Press

Language:

English

Submitter:

Marceline Brodmann

Date Deposited:

14 Jul 2020 16:07

Last Modified:

24 Jul 2020 19:03

Publisher DOI:

10.1017/S0031182000084730

PubMed ID:

8851856

BORIS DOI:

10.7892/boris.115443

URI:

https://boris.unibe.ch/id/eprint/115443

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