The Role of Formylpeptide Receptors, C5a Receptors, and Cytosolic-Free Calcium in Neutrophil Priming

Zimmerli, Werner; Reber, Anne-Marie; Dahinden, Clemens A. (1990). The Role of Formylpeptide Receptors, C5a Receptors, and Cytosolic-Free Calcium in Neutrophil Priming. Journal of infectious diseases, 161(2), pp. 242-249. The University of Chicago Press 10.1093/infdis/161.2.242

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Polymorphonuclear leukocytes (PMNL) exposed to chemoattractants or cytokines change their functional capacity. The effect of endotoxin-activated serum as a priming agent on human PMNL was tested. Pretreatment of PMNL with endotoxin-activated serum increased their oxidative burst in response to formylpeptide (FMLP) (P<.02)and CSa (P<.05). Priming for membrane depolarization was observed in PMNL preincubated with either endotoxin-activated serum, low concentrations of purified C5a, or endotoxin but not with decomplemented plasma. Primed PMNL had an increased number of FMLP but not C5a receptors as compared with control PMNL. The “resting” cytosolic free calcium was increased in primed PMNL (P<.02). Intracellular calcium buffering abolished the priming effect of endotoxin-activated serum. Thus, endotoxin-activated serum can prime cellular responsiveness for membrane depolarization and superoxide production in response to FMLP and to C5a. Priming may be due to an increased resting cytosolic-free calcium.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute for Immunology [discontinued]

UniBE Contributor:

Dahinden, Clemens A.

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0022-1899

Publisher:

The University of Chicago Press

Language:

English

Submitter:

Marceline Brodmann

Date Deposited:

08 Oct 2020 08:38

Last Modified:

08 Oct 2020 08:38

Publisher DOI:

10.1093/infdis/161.2.242

BORIS DOI:

10.7892/boris.115585

URI:

https://boris.unibe.ch/id/eprint/115585

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