GC-MS Determination of Flunitrazepam and its Major Metabolite in Whole Blood and Plasma

Elsohly, M.A.; Feng, S.; Salamone, S.J.; Brenneisen, R. (1999). GC-MS Determination of Flunitrazepam and its Major Metabolite in Whole Blood and Plasma. Journal of analytical toxicology, 23(6), pp. 486-489. Oxford University Press 10.1093/jat/23.6.486

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A gas chromatography-mass spectrometry method was developed for the analysis of flunitrazepam (FN) and its major metabolite, 7-amino-flunitrazepam (7-amino-FN), in both plasma and whole blood. The method was based on acid hydrolysis of the samples after dilution with HPLC water followed by extraction and derivatization (heptafluorobutyrate) of the resulting benzophenones. Analysis of plasma and whole blood samples from subjects administered 2-mg doses of FN showed that FN was only detected in whole blood (LOD 5 ng/mL) and not in plasma. However, 7-Amino-FN was detected in both plasma and whole blood, although the levels were much higher in plasma. 7-Amino-FN was detected for the entire period of specimen collection (12 h), but FN was only detected in whole blood for 4 h after ingestion with peak levels after 1 h.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Phytopharmakologie, Bioanalytik & Pharmakokinetik [discontinued]
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Phytopharmakologie, Bioanalytik & Pharmakokinetik [discontinued]

UniBE Contributor:

Brenneisen, Rudolf Max

ISSN:

0146-4760

Publisher:

Oxford University Press

Language:

English

Submitter:

Marceline Brodmann

Date Deposited:

02 Sep 2020 16:36

Last Modified:

02 Sep 2020 16:57

Publisher DOI:

10.1093/jat/23.6.486

BORIS DOI:

10.7892/boris.115624

URI:

https://boris.unibe.ch/id/eprint/115624

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