Vaudaux, Pierre; Pittet, Didier; Häberli, André; Lerch, Peter G.; Morgenthaler, Jean-Jacques; Proctor, Richard A.; Waldvogel, Francis A.; Lew, Daniel P. (1993). Fibronectin Is More Active than Fibrin or Fibrinogen in Promoting Staphylococcus aureus Adherence to Inserted Intravascular Catheters. Journal of infectious diseases, 167(3), pp. 633-641. The University of Chicago Press 10.1093/infdis/167.3.633
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To further define the role of fibrin(ogen) and fibronectin in Staphylococcus aureus adherence to central venous catheters, the amount, chemical integrity, and biologic activity of these proteins adsorbed on lines inserted in hospitalized patients were prospectively studied. Polyurethane cannulas promoted a significantly lower adherence of S. aureus than polyvinyl chloride (P < .01) or Hickman (P < .001) cannulas and contained the lowest amount of immunologically assayed fibronectin but not of fibrin(ogen). Fibrinogen showed an extensive loss of adherence-promoting activity on inserted cannulas, which was related to its proteolytic breakdown, as detected by SDS-PAGE and immunoblots with antifibrinogen antibodies and confirmed by in vitro studies with purified protein fragments. In contrast, either intact or fragmented fibronectin, although present in much lower amounts than fibrin(ogen), could actively promote S. aureus adherence onto intravenous catheters.
Item Type: |
Journal Article (Original Article) |
|---|---|
UniBE Contributor: |
Häberli, André |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
0022-1899 |
Publisher: |
The University of Chicago Press |
Language: |
English |
Submitter: |
Marceline Brodmann |
Date Deposited: |
15 Oct 2020 12:08 |
Last Modified: |
05 Dec 2022 15:13 |
Publisher DOI: |
10.1093/infdis/167.3.633 |
BORIS DOI: |
10.7892/boris.115837 |
URI: |
https://boris.unibe.ch/id/eprint/115837 |
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