Differential Regulation of Inducible Nitric Oxide Synthase Production in Bovine and Caprine Macrophages

Adler, Heiko; Adler, Barbara; Peveri, Paola; Werner, Ernst R.; Wachter, Helmut; Peterhans, Ernst; Jungi, Thomas W. (1996). Differential Regulation of Inducible Nitric Oxide Synthase Production in Bovine and Caprine Macrophages. Journal of infectious diseases, 173(4), pp. 971-978. The University of Chicago Press 10.1093/infdis/173.4.971

[img]
Preview
Text
173-4-971.pdf - Published Version
Available under License Publisher holds Copyright.

Download (871kB) | Preview

Inducible nitric oxide synthase (iNOS) regulation in human and murine macrophages in vitro differs considerably. In this study, expression of macrophage iNOS in ruminants was addressed. Nitric oxide (NO) output by cattle and goat macrophages was as different as that by human and mouse macrophages. Bovine macrophages activated by heated Salmonella dublin or lipopolysaccharide (LPS) expressed high levels of iNOS mRNA, protein, and enzyme activity. Analogously cultured caprine macrophages did not respond to these and other activators by NO generation and iNOS expression. The lack of response was not due to general unresponsiveness to stimuli. Caprine iNOS mRNA was induced by stimulation of caprine macrophages with LPS, as shown by reverse transcription polymerase chain reaction. The level of mRNA expression in activated goat macrophages was lower than in resting bovine macrophages. A caprine 372-bp iNOS mRNA fragment that was sequenced closely resembled the bovine counterpart. This points to species-specific iNOS gene regulation.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology

UniBE Contributor:

Peterhans, Ernst and Jungi, Thomas

Subjects:

600 Technology > 630 Agriculture

ISSN:

0022-1899

Publisher:

The University of Chicago Press

Language:

English

Submitter:

Marceline Brodmann

Date Deposited:

08 Oct 2020 09:03

Last Modified:

08 Oct 2020 09:03

Publisher DOI:

10.1093/infdis/173.4.971

BORIS DOI:

10.7892/boris.116056

URI:

https://boris.unibe.ch/id/eprint/116056

Actions (login required)

Edit item Edit item
Provide Feedback