Waterlogging affects plant morphology and the expression of key genes in tef ( Eragrostis tef )

Cannarozzi, Gina Michelle; Weichert, Annett; Schnell, Mirjam; Ruiz, Celia; Bossard, Svenja; Blösch, Regula; Plaza-Wüthrich, Sonia; Chanyalew, Solomon; Assefa, Kebebew; Tadele, Zerihun (2018). Waterlogging affects plant morphology and the expression of key genes in tef ( Eragrostis tef ). Plant direct, 2(4), e00056. Wiley 10.1002/pld3.56

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Tef [Eragrostis tef (Zucc.) Trotter], an allotetraploid cereal that is a staple food to over 60 million people in the Horn of Africa, has a high nutritional content and is resistant to many biotic and abiotic stresses such as waterlogging and drought. Three tef genotypes, Alba, Tsedey, and Quncho, were subjected to waterlogging conditions and their growth, physiology, and change in transcript expression were measured with the goal of identifying targets for breeding cultivars with improved waterlogging tolerance. Root and shoot growth and dry weight were observed over 22 days. Stomatal conductance and chlorophyll and carotenoid contents were quantified. Microscopy was used to monitor changes in the stem cross sections. Illumina RNA sequencing was used to obtain the expression profiles of tef under flooding and control conditions and was verified using qPCR. Results indicated differences in growth between the three genotypes. Waterlogged Tsedey plants grew higher and had more root biomass than normally watered Tsedey plants. Quncho and Alba genotypes were more susceptible to the excess moisture stress. The effects of these changes were observed on the plant physiology. Among the three tested tef genotypes, Tsedey formed more aerenchyma than Alba and had accelerated growth under waterlogging. Tsedey and Quncho had constitutive aerenchyma. Genes affecting carbohydrate metabolism, cell growth, response to reactive oxygen species, transport, signaling, and stress responses were found to change under excess moisture stress. In general, these results show the presence of substantial anatomical and physiological differences among tef genotypes when waterlogged during the early growth stage.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Cannarozzi, Gina Michelle; Weichert, Annett; Schnell, Mirjam; Blösch, Regula; Plaza, Sonia and Tadele, Zerihun

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

2475-4455

Publisher:

Wiley

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

08 May 2018 15:13

Last Modified:

23 Oct 2019 02:27

Publisher DOI:

10.1002/pld3.56

Uncontrolled Keywords:

adventitious roots; aerenchyma; differential expression, Eragrostis tef; flooding; grass family; RNA‐Seq; tef; transcriptome; waterlogging

BORIS DOI:

10.7892/boris.116197

URI:

https://boris.unibe.ch/id/eprint/116197

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