Cell Type-Specific Targeting Strategies for Optogenetics

Yizhar, Ofer; Adamantidis, Antoine Roger (2018). Cell Type-Specific Targeting Strategies for Optogenetics. In: Stroh, Albrecht (ed.) Optogenetics: A Roadmap. Neuromethods: Vol. 133 (pp. 25-42). New York, NY: Humana Press 10.1007/978-1-4939-7417-7_2

Full text not available from this repository. (Request a copy)

Abstract View references (89) Optogenetic techniques allow versatile, cell type-specific light-based control of cellular activity in diverse set of cells, circuits, and brain structures. Optogenetic actuators are genetically encoded light-sensitive membrane proteins that can be selectively introduced into cellular circuits in the living brain using a variety of genetic approaches. Gene targeting approaches used in optogenetic studies vary greatly in their specificity, their spatial coverage, the level of transgene expression and their potential adverse effects on neuronal cell health. Here, we describe the major gene targeting approaches utilized in optogenetics and provide a simple set of guidelines through which these approaches can be evaluated when designing an in vitro or in vivo optogenetic study. © Springer Science+Business Media LLC 2018.

Item Type:

Book Section (Book Chapter)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurology

UniBE Contributor:

Adamantidis, Antoine Roger

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1940-6045

ISBN:

978-1-4939-7415-3

Series:

Neuromethods

Publisher:

Humana Press

Language:

English

Submitter:

Stefanie Hetzenecker

Date Deposited:

09 May 2018 12:49

Last Modified:

09 May 2018 12:49

Publisher DOI:

10.1007/978-1-4939-7417-7_2

URI:

https://boris.unibe.ch/id/eprint/116238

Actions (login required)

Edit item Edit item
Provide Feedback