Electrospinning of highly concentrated albumin patches by using auxiliary polymers for laser-assisted vascular anastomosis.

Schönfeld, Annemarie; Constantinescu, Mihai Adrian; Peters, Kirsten; Frenz, Martin (2018). Electrospinning of highly concentrated albumin patches by using auxiliary polymers for laser-assisted vascular anastomosis. Biomedical materials, 13(5), 055001. Institute of Physics Publishing IOP 10.1088/1748-605X/aac332

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BACKGROUND AND OBJECTIVE Electrospun meshes have been extensively investigated for tissue engineering and drug delivery. The application of this technology is of interest for laser-assisted vascular anastomosis (LAVA) due to the possibility to bind and stabilize macromolecules in fibers.
 Materials and Methods: We prepared bovine serum albumin (BSA) blend microfibers from the auxiliary proteins polyethylene oxide (PEO), polycaprolactone (PCL), polyvinyl alcohol (PVA) and gelatin. The thickness and weight of the resulting patches were measured and the morphological characteristics were observed by scanning electron microscopy. Thereafter, layered patches were prepared by spinning the BSA/polymer layer on top of a light-absorbing layer made of indocyanine green and PCL. The effect of the material composition of the electrospun patches on the behavior during LAVA, the bonding strength and the resulting thermal damage were investigated.
 Results: The bonding strength of the tissue fusion increased with higher BSA amounts in the patch. By using PEO, a ratio of 85/15 (w/w) of BSA/PEO was stable during electrospinning, leading to a shear strength that was similar to patches that were soaked in liquid BSA (20.7±4.1mN/mm2 and 20.3±4.1mN/mm2, respectively). The handling during LAVA was however drastically improved by using a layered patch made from BSA/PEO. Thermal damage was similar compared to previous solder materials. 
 Conclusion: This study investigated the maximum amount of BSA possible in electrospun polymer fibers made from PEO, PCL, PVA and gelatin. Both, the process of electrospinning and the performance during ex vivo LAVA, makes the BSA/PEO blend a promising material for LAVA.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Plastic and Hand Surgery > Plastic, Reconstructive and Aesthetic Surgery
08 Faculty of Science > Institute of Applied Physics
04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Plastic and Hand Surgery

UniBE Contributor:

Constantinescu, Mihai Adrian and Frenz, Martin

Subjects:

600 Technology > 620 Engineering
600 Technology > 610 Medicine & health

ISSN:

1748-6041

Publisher:

Institute of Physics Publishing IOP

Language:

English

Submitter:

Veronika Picha

Date Deposited:

14 May 2018 14:31

Last Modified:

10 May 2019 02:30

Publisher DOI:

10.1088/1748-605X/aac332

PubMed ID:

29739918

Uncontrolled Keywords:

in vitro studies indocyanine green shear strength tissue fusion tissue soldering

BORIS DOI:

10.7892/boris.116521

URI:

https://boris.unibe.ch/id/eprint/116521

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