BRU1, a novel link between responses to DNA damage and epigenetic gene silencing in Arabidopsis

Takeda, Shin; Tadele, Zerihun; Hofmann, Ingo; Probst, Aline V.; Angelis, Karel J.; Kaya, Hidetaka; Araki, Takashi; Mengiste, Tesfaye; Scheid, Ortrun Mittelsten; Shibahara, Kei-ichi; Scheel, Dierk; Paszkowski, Jerzy (2004). BRU1, a novel link between responses to DNA damage and epigenetic gene silencing in Arabidopsis. Genes & development, 18(7), pp. 782-793. Cold Spring Harbor Laboratory Press 10.1101/gad.295404

[img]
Preview
Text
2004_GenesDev_18_782.pdf - Published Version
Available under License Publisher holds Copyright.

Download (391kB) | Preview

DNA repair associated with DNA replication is important for the conservation of genomic sequence information, whereas reconstitution of chromatin after replication sustains epigenetic information. We have isolated and characterized mutations in the BRU1 gene of Arabidopsis that suggest a novel link between these underlying maintenance mechanisms. Bru1 plants are highly sensitive to genotoxic stress and show stochastic release of transcriptional gene silencing. They also show increased intrachromosomal homologous recombination and constitutively activated expression of poly (ADP-ribose) polymerase-2 (AtPARP-2), the induction of which is associated with elevated DNA damage. Bru1 mutations affect the stability of heterochromatin organization but do not interfere with genome-wide DNA methylation. BRU1 encodes a novel nuclear protein with two predicted protein–protein interaction domains. The developmental abnormalities characteristic of bru1 mutant plants resemble those triggered by mutations in genes encoding subunits of chromatin assembly factor (CAF-1), the condensin complex, or MRE11. Comparison of bru1 with these mutants indicates cooperative roles in the replication and stabilization of chromatin structure, providing a novel link between chromatin replication, epigenetic inheritance, S-phase DNA damage checkpoints, and the regulation of meristem development.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Tadele, Zerihun

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0890-9369

Publisher:

Cold Spring Harbor Laboratory Press

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

14 Jun 2018 11:08

Last Modified:

14 Jun 2018 11:08

Publisher DOI:

10.1101/gad.295404

Uncontrolled Keywords:

Arabidopsis thaliana; DNA repair; epigenetic inheritance; gene silencing; homologous recombination

BORIS DOI:

10.7892/boris.116532

URI:

https://boris.unibe.ch/id/eprint/116532

Actions (login required)

Edit item Edit item
Provide Feedback