High-resolution capillary zone electrophoresis for transferrin glycoform analysis associated with congenital disorders of glycosylation.

Tobler, Micha; Caslavsky, Jitka; Burda, Patricie; Thormann, Wolfgang (2018). High-resolution capillary zone electrophoresis for transferrin glycoform analysis associated with congenital disorders of glycosylation. Journal of separation science, 41(13), pp. 2808-2818. Wiley-VCH 10.1002/jssc.201800082

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High-resolution capillary zone electrophoresis is used to assess the transferrin profile in serum of patients with eight different congenital disorders of glycosylation that represent type I, type II and mixed type I/II disorders. Capillary zone electrophoresis data are compared to patterns obtained by gel isoelectric focusing. The high-resolution capillary zone electrophoresis method is shown to represent an effective tool to assess the diversity of transferrin patterns. Hypoglycosylated disialo-, monosialo- and asialo-transferrin in type I cases can be distinguished from the corresponding underdesialylated transferrin glycoforms present in type II disorders. The latter can be separated from and are detected ahead of their corresponding hypoglycosylated forms of type I patients. Both types of glycoforms are detected in sera of mixed type I/II patients. The assay has the potential to be used as screening method for congenital disorders of glycosylation. It can be run with a few μL of serum when microvials are used. This article is protected by copyright. All rights reserved.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Laboratory for Clinical Pharmacology

UniBE Contributor:

Caslavsky, Jitka and Thormann, Wolfgang

ISSN:

1615-9306

Publisher:

Wiley-VCH

Language:

English

Submitter:

Wolfgang Thormann

Date Deposited:

28 May 2018 16:07

Last Modified:

28 Apr 2019 02:30

Publisher DOI:

10.1002/jssc.201800082

PubMed ID:

29701302

Additional Information:

Micha Tobler and Jitka Caslavska contributed equally to this work.

Uncontrolled Keywords:

capillary electrophoresis congenital disorder of glycosylation desialylated transferrin transferrin glycoforms

BORIS DOI:

10.7892/boris.116602

URI:

https://boris.unibe.ch/id/eprint/116602

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