High-throughput annotation of full-length long noncoding RNAs with capture long-read sequencing.

Lagarde, Julien; Uszczynska-Ratajczak, Barbara; Carbonell, Silvia; Pérez-Lluch, Sílvia; Abad, Amaya; Davis, Carrie; Gingeras, Thomas R; Frankish, Adam; Harrow, Jennifer; Guigo, Roderic; Johnson, Rory (2017). High-throughput annotation of full-length long noncoding RNAs with capture long-read sequencing. Nature genetics, 49(12), pp. 1731-1740. Nature America 10.1038/ng.3988

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Accurate annotation of genes and their transcripts is a foundation of genomics, but currently no annotation technique combines throughput and accuracy. As a result, reference gene collections remain incomplete-many gene models are fragmentary, and thousands more remain uncataloged, particularly for long noncoding RNAs (lncRNAs). To accelerate lncRNA annotation, the GENCODE consortium has developed RNA Capture Long Seq (CLS), which combines targeted RNA capture with third-generation long-read sequencing. Here we present an experimental reannotation of the GENCODE intergenic lncRNA populations in matched human and mouse tissues that resulted in novel transcript models for 3,574 and 561 gene loci, respectively. CLS approximately doubled the annotated complexity of targeted loci, outperforming existing short-read techniques. Full-length transcript models produced by CLS enabled us to definitively characterize the genomic features of lncRNAs, including promoter and gene structure, and protein-coding potential. Thus, CLS removes a long-standing bottleneck in transcriptome annotation and generates manual-quality full-length transcript models at high-throughput scales.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Medical Oncology

UniBE Contributor:

Johnson, Rory Baldwin

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1061-4036

Publisher:

Nature America

Language:

English

Submitter:

Nicole Corminboeuf

Date Deposited:

14 Jun 2018 10:13

Last Modified:

22 Jun 2018 14:48

Publisher DOI:

10.1038/ng.3988

PubMed ID:

29106417

BORIS DOI:

10.7892/boris.116920

URI:

https://boris.unibe.ch/id/eprint/116920

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