Plasmodium gametocytes display homing and vascular transmigration in the host bone marrow.

De Niz, Mariana; Meibalan, Elamaran; Mejia, Pedro; Ma, Siyuan; Brancucci, Nicolas M B; Agop-Nersesian, Carolina; Mandt, Rebecca; Ngotho, Priscilla; Hughes, Katie R; Waters, Andrew P; Huttenhower, Curtis; Mitchell, James R; Martinelli, Roberta; Frischknecht, Friedrich; Seydel, Karl B; Taylor, Terrie; Milner, Danny; Heussler, Volker T.; Marti, Matthias (2018). Plasmodium gametocytes display homing and vascular transmigration in the host bone marrow. Science Advances, 4(5), eaat3775. American Association for the Advancement of Science 10.1126/sciadv.aat3775

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Transmission of Plasmodium parasites to the mosquito requires the formation and development of gametocytes. Studies in infected humans have shown that only the most mature forms of Plasmodium falciparum gametocytes are present in circulation, whereas immature forms accumulate in the hematopoietic environment of the bone marrow. We used the rodent model Plasmodium berghei to study gametocyte behavior through time under physiological conditions. Intravital microscopy demonstrated preferential homing of early gametocyte forms across the intact vascular barrier of the bone marrow and the spleen early during infection and subsequent development in the extravascular environment. During the acute phase of infection, we observed vascular leakage resulting in further parasite accumulation in this environment. Mature gametocytes showed high deformability and were found entering and exiting the intact vascular barrier. We suggest that extravascular gametocyte localization and mobility are essential for gametocytogenesis and transmission of Plasmodium to the mosquito.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology > Malaria
08 Faculty of Science > Department of Biology > Institute of Cell Biology
09 Interdisciplinary Units > Microscopy Imaging Center MIC

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Heussler, Volker

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

2375-2548

Publisher:

American Association for the Advancement of Science

Funders:

[42] Schweizerischer Nationalfonds
[81] Evimalar

Language:

English

Submitter:

Volker Heussler

Date Deposited:

08 Jun 2018 11:33

Last Modified:

23 Oct 2019 13:32

Publisher DOI:

10.1126/sciadv.aat3775

PubMed ID:

29806032

BORIS DOI:

10.7892/boris.117074

URI:

https://boris.unibe.ch/id/eprint/117074

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