Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures.

Zhang, Feng; Gradinaru, Viviana; Adamantidis, Antoine Roger; Durand, Remy; Airan, Raag D; de Lecea, Luis; Deisseroth, Karl (2010). Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures. Nature protocols, 5(3), pp. 439-456. Nature Publishing Group 10.1038/nprot.2009.226

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Elucidation of the neural substrates underlying complex animal behaviors depends on precise activity control tools, as well as compatible readout methods. Recent developments in optogenetics have addressed this need, opening up new possibilities for systems neuroscience. Interrogation of even deep neural circuits can be conducted by directly probing the necessity and sufficiency of defined circuit elements with millisecond-scale, cell type-specific optical perturbations, coupled with suitable readouts such as electrophysiology, optical circuit dynamics measures and freely moving behavior in mammals. Here we collect in detail our strategies for delivering microbial opsin genes to deep mammalian brain structures in vivo, along with protocols for integrating the resulting optical control with compatible readouts (electrophysiological, optical and behavioral). The procedures described here, from initial virus preparation to systems-level functional readout, can be completed within 4-5 weeks. Together, these methods may help in providing circuit-level insight into the dynamics underlying complex mammalian behaviors in health and disease.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurology

UniBE Contributor:

Adamantidis, Antoine Roger

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1754-2189

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Stefanie Hetzenecker

Date Deposited:

11 Jul 2018 16:48

Last Modified:

11 Jul 2018 16:48

Publisher DOI:

10.1038/nprot.2009.226

PubMed ID:

20203662

URI:

https://boris.unibe.ch/id/eprint/117260

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